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ID 10572
Eprint ID
10572
FullText URL
Thumnail 93_19_27.pdf 1.63 MB
Title Alternative
ピーマン(Capsicum annuum L.)に導入されたL2抵抗性遺伝子を打破する日本産トバモウイルス系統ペッパーマイルドモットルウイルス(PMMoV)の疫学的調査
Author
Toyoda, Kazuhiro Kakenhi
Hikichi, Yasufumi
Takeuchi, Shigeharu
Kuroda, Tomohisa
Okumura, Ako
Nasu, Yoshiko
Okuno, Tetsuro
Suzuki, Kazumi
Abstract
To understand the epidemiological aspects of tobamovirus infecting the L resistance genotypes of green pepper, fifteen isolates were collected from geographically different fields and were chracterized by their biological properties. All isolates infected L1 and L2 plants systemically, but were localized in L3 and L4 plants. The symptomatology on several test plants and the reactivity to an antiserum showed that they were identical to that of a Japanese strain of pepper mild mottle virus (PMMoV-J). The viral infection was also confirmed by a reverse transcription and polymerase chain reaction (RT-PCR) with oligonucleotide primers that amplity the coat protein gene of PMMoV-RNA. On the other hand, the RT-PCR allowed us to detect PMMoV in seeds of some commercial cultivars of green pepper. Viruses isolated from the seeds could infect L2 plants systemically. Further analysis of the nucleotide sequence of the predicted coat protein gene revealed that the isolates from the commercial seeds were identical to that of PMMoV-J. These results indicated that the L2 resistance-breaking tobamovirus has prevailed in fields of green pepper in Japan. and that infected seeds may be one of the initial sources of the viral infection.
Keywords
Capsicum annuum L.
Pepper mild mottle virus(PMMoV)
PT-PCR
resistance-breaking tobamovirus
Published Date
2004-02
Publication Title
岡山大学農学部学術報告
Publication Title Alternative
Scientific reports of the Faculty of Agriculture, Okayama University
Volume
volume93
Issue
issue1
Publisher
岡山大学農学部
Publisher Alternative
Faculty of Agriculture,Okayama University
Start Page
19
End Page
27
ISSN
0474-0254
NCID
AN00033029
Content Type
Departmental Bulletin Paper
language
英語
File Version
publisher
Refereed
False
Eprints Journal Name
srfa