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ID 11069
Eprint ID
11069
FullText URL
Title Alternative
Stabilization of cyclo (Gly-Tyr)-hydrolyzing Enzyme by Synthetic Glycoside-bearing Polymer
Author
Abe, Yasuteru
Kawazu, Kazuyoshi
Abstract
An enrichment technique led us to isolate 13 cyclo (Gly-Tyr)-assimilating bacteria, which had higher cyclo (Gly-Tyr)-hydrolyzing activity that the coryneform rod bacterium strain T-1-3-Y,previously isolated from soil. The cell-free extract of the strain Ol-L-2 prepared by ultrasonication in a buffer containing bovine serum albumin had cyclo (Gly-Tyr)-hydrolyzing activity while that of the strain T-1-3-Y showed no activity. GEMA (Glucosyloxyethyl methacrylate) polymer was found to be a more effective stabilizer for solubilizing cyclo (Gly-Tyr) hydrolase than bovine serum albumin.
Abstract Alternative
集積培養法を用いて新たに土壌よりcyclo(Gly-Tyr)資化性菌を分離し、その中から、従来の資化性菌より菌体当たりの活性の高い菌株を13株見いだした。これらの菌株を牛血清アルブミンを添加した緩衝液中に懸濁し、超音波破砕に供したところ、OI-L-2株由来の無細胞抽出液中にcyclo(Gly-Tyr)分解活性が検出された。この活性は安定化剤をアルブミンから配糖化合成高分子GEMAポリマーに代えることによりさらに安定に抽出された。
Keywords
diketopiperazine
cyclic dipeptide
hydrolysis
protease
Published Date
1998-02
Publication Title
岡山大学農学部学術報告
Publication Title Alternative
Scientific reports of the Faculty of Agriculture, Okayama University
Volume
volume87
Issue
issue1
Publisher
岡山大学農学部
Publisher Alternative
Faculty of Agriculture,Okayama University
Start Page
43
End Page
46
ISSN
0474-0254
NCID
AN00033029
Content Type
Departmental Bulletin Paper
language
日本語
File Version
publisher
Refereed
False
Eprints Journal Name
srfa