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ID 13577
Eprint ID
13577
FullText URL
114_289.pdf 2.99 MB
Title Alternative
Effect of phospholipase A(2) on proliferation of the primary cultured biliary epithelial cells from the extra-hepatic bile duct to rats using D-valine containing medium
Author
Ogura, Kaoru
Watanabe, Yasuhiro
Hiramine, Chiharu
Nakagawa, Toshitaka
Toki, Akira
Tokuda, Masaaki
Abstract
We hypothesized that a high level of phospholipase A(2) (PLA(2)) in bile juice works as a growth factor of biliary epithelial cells and causes hyperplastic change of the bile duct in pancreaticobiliary maljunction. in order to examine this hypothesis, we established a primary culture system of biliary epithelial cells from the extra-hepatic bile duct of rats. We successfully excluded fibroblasts from the epithelial cells using D-valine-containing medium and the single-cell cloning method. The established cells exhibited the characteristic features of rat bile duct epithelial cells. They were stained with anti-cytokeratin antibody, a marker protein of epithelial cells, were positive with PAS stain, and possessed the architectures of microvilli, tonofilament fibers and desmosomes. The effect of PLA(2) on proliferation of these cells was then examined. We clearly demonstrated that PLA(2) at nano-molar concentration (50nM, 100nM), that is, equivalent to the level of PLA(2) in the bile juice of pancreaticobiliary maljunction cases, caused a significant enhancement of epithelial cell proliferation.
Keywords
膵・胆管合流異常
胆管上皮細胞
D-バリン
Phospholipase A(2)
細胞増殖
Note
原著論文
Published Date
2003-01-31
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume114
Issue
issue3
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
289
End Page
296
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/114/3/114_3_289/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/index.html
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma