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ID 13589
Eprint ID
13589
FullText URL
Thumnail 113_247.pdf 4.04 MB
Title Alternative
Expression of amphiphysin Ⅰ, a nerve terminal-enriched protein, in the chromaffin cells of the rat adrenal medulla
Author
Okamoto, Yoko
Abstract
Amphiphysin (amph)Ⅰ, a protein concentrated in the nerve terminals, plays an essential role in clathrin-mediated endocytosis of synaptic vesicle membrane through an interaction with GTP ase dynamin. A speciffic antibody against amph Ⅰ revealed that the protein was also expressed in adrenal medullary cells. In the postnatal 11th day (P11) and adult rats, combined fluorescence histochemistry for catecholamine-containing cells with immunohistochemistry for amph Ⅰ disclosed strong and weak immunochemical signal of amph Ⅰ in noradrenalin (NA)- and adrenalin (A)-storing cells, respectively. Very strong immunoreactivity to amph Ⅰ was found in the intramedullary neurons but not in the adrenal cortex. In developmental stages, amph Ⅰ was discernible in the medullary chromaffin cells on 16th embryonic day (E16), E19, P0 and P2. Dopamin-β―hydroxylase (a catecholamine-synthesizing enzyme converting dopamin to noradrenalin) was immunohistochemically detected in the chromaffin cells during the parinatal periods. Colocalization of amph Ⅰ with dynamin was seen in NA-cells but not in A-cells. The present findings suggest that amph Ⅰ in the adrenal medullary NA-and A-storing cells is involved in membraine recycling mechanisms, which may be unique to each cell type, through endocytic activities.
Keywords
ラット副腎髄質
クロマフィン細胞
amphiphysin Ⅰ
免疫組織化学
Note
原著
Published Date
2001-12-31
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume113
Issue
issue3
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
247
End Page
259
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/113/3/113_3_247/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/index.html
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma