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ID 14586
Eprint ID
14586
FullText URL
Thumnail 078_317.pdf 4.18 MB
Author
Kotsuka, Takashi
Abstract
50 cases of leukemic bone marrow were studied by the fluorochrominized tissue culture with acridine orange divised in our laboratory. Following results were obtained. 1. In acute leukemias, the growth area was extremely dense with migrating cells and its boundary was distinctly defined. It was characteristic in fluorochrominized tissue culture that the greater part of the growth area was green in color owing to the fluorescence of blast cells and its outer zone was somewhat reddish in acute myelogenous leukemia and yellowish in acute lymphocytic leukemia because of the presence of mature cells. 2. In chronic leukemias, the well-defined boundary of the growth area with a high cell density, which was also characteristic of acute leukemia, was sorrounded by another less dense zone of mature cells, forming a double growth zone. In chronic myelogenous leukemia, the inner zone of this doudle growth zone showed reddish green color, and in chronic lymphocytic leukemia yellowish green color. In the outer zone of this double growth zone the flnorescence of mature leukocytes was especially predominant. 3. In monocytic leukemia, the growth pattern was almost similar to that in aute lenkemia, but its fluorescent color was somewhat different. In the growth area there were many, greenish orange colored promonocytes and monocytes, green monoblasts, yellow lymphocytes and a few neutrophils with reddish orange granules. Therefore the growth area in monocytic leukemia disclosed multi-colored fluorescence. 4. To conclude these results, the growth area of each type of leukemia were occupied by the fluorescence of predominant leukemic cells. Accordingly by the fluorochrominized tissue culture of bone marrow the diagnosis of leukemia types could be performed.
Note
学位番号 乙第172号
Published Date
1966-03-30
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume78
Issue
issue2-3
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
317
End Page
329
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/78/2-3/78_2-3_317/_article/-char/ja/
Related Url
http://eprints.lib.okayama-u.ac.jp/3822/
http://eprints.lib.okayama-u.ac.jp/14587/
language
日本語
OAI-PMH Set
岡山大学
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma