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ID 17001
Eprint ID
17001
FullText URL
Thumnail 92_237.pdf 398 KB
Title Alternative
Immunoreactive dog C-peptide levels in pancreatic vein Part I: Dog C-peptide radioimmunoassay and C-peptide immunoreactivity in pancreatic vein after loading of glucose and arginine
Author
Nishina, Yoshiaki
Abstract
A specific dog C-peptide radioimmunoassay system was established with a single antibody method using synthetic dog C-peptide as standard. The measurable range was between 0.4 pM/ml and 25.6 pM/ml. The coefficients of variation of Within and Between-assay were 6.0 and 10.8%, respectively. Dog C-peptide immunoreactivity (CPR) and immunoreactive insulin (IRI) level in the superior pancreaticoduodenal (pancreatic) vein were measured in fasting and after loading of glucose or arginine into the superior pancreaticoduodenal (pancreatic) artery. Fasting dog CPR level was 2.73 ± 0.46 pM/ml in the pancreatic vein and was 0.67 ± 0.03 pM/ml in peripheral vein. The molar ratio of CPR/IRI was 1.42 in the pancreatic vein and 6.09 in peripheral vein. Glucose (10 mg/kg/min) infusion into the pancreatic artery evoked a prompt and parallel maximal increase in CPR and IRI within 3 min. The CPR concentration increased from 3.1 pM/ml to 6.5 pM/ml and the IRI concentration increased from 1.7 pM/ml to 4.6 pM/ml at the peak. Arginine (250 mg/kg/min) infusion resulted in a prompt, parallel increase in CPR and IRI. The CPR concentration increased from 3.2 pM/ml to 5.8 pM/ml, and the IRI concentration from 1.7 pM/ml to 4.4 pM/ml at 40 sec, respectively. The insulin - to - C-peptide ratio showed approximately equimolarity.
Keywords
イヌC-ペプチドラジオイムノアッセイ
イヌ膵静脈血中C-ペプチド濃度(CPR)
イヌ膵静脈血中インスリン濃度(IRI)
Published Date
1980-02-28
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume92
Issue
issue1-2
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
237
End Page
245
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/92/1-2/92_1-2_237/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/index.html
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma