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ID 17077
Eprint ID
17077
FullText URL
Thumnail 89_185.pdf 465 KB
Title Alternative
Study on the hypoplastic anemia. Part Ⅱ. Studies on the lymphocytes of the patients with aplastic anemia
Author
Mituhashi, Asako
Abstract
Twenty-five patients with aplastic anemia, 8 female and 17 male aged from 16 to 79 year-old, were examined their circulating lymphocyte subpopulations and the responses to mitogens in vitro.T-lymphocyte was identified using sheep erythrocyte rosette-formation test under the Nikon phase contrast microscope. Surface immunoglobulin bearing cell was identified under the Nikon fluoromicroscope as B-lymphocyte using membrane immuno-fluorescence test. As mitogens in vitro PHA-P (Difco Lab.) and pokeweed mitogen (Grand Island Biol. Co.) were added to the lymphocyte culture during 68 hours in the CO(2) incubator following 4 hours flushing with (3)H-TdR. The incorporated cpm was counted with millipore filtration and a liquid scintillation counter (Shimazu). According the severity of aplastic anemia, the patients showed decreased circulating lymphocytes, decreased T- and B-lymphocytes with increased null cells. The incorporation of (3)H-TdR in vitro in the soloculture decreased remarkably as well as PHA-P responsiveness. Increased bone marrow T-lymphosytes were noticed in those patients. Although the higher cpm of soloculture of the bone marrow lymphocyte was obtained than of the circulating lymphocyte, extremely low responses to PHA-P and pokeweed mitogen were characteristic in the aplastic bone marrow lymphocytes. The splenectomy corrected T- and B-lymphocytes population in contrast to the persistent low responsiveness to mitogens. The abnormality of lymphocyte subpopulations and incorporation rate of (3)H-thymidin stimulated with pokeweed mitogen and PHA-P might effect on bone marrow cells as cytotoxic or cytolytic.
Published Date
1977-02-28
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume89
Issue
issue1-2
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
185
End Page
194
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/89/1-2/89_1-2_185/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/index.html
language
日本語
OAI-PMH Set
岡山大学
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma