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ID 17434
Eprint ID
17434
FullText URL
Thumnail 87_543.pdf 235 KB
Title Alternative
Denaturation of Catalase in Livers by SDS Treatment
Author
Mizugaki, Junko
Abstract
When the crystalline beef liver catalase was treated with SDS (sodium n-dodecyl sulfate) or LIS (3, 5-diiodosalicylic acid lithium salt) which is a kind of the anionic surfactant, its catalase activity dwindled and disappeared at last, and its peroxidatic activity was exhibited. It was found by the ultracentrifugal analysis that the molecule of beef liver catalase treated with SDS splited into two subunits with sedimentation constants of about 10S and 2S. The same phenomena as mentioned above were compared in the crude catalase extracts of livers from normal mice and acatalasemia mice. In the crude catalase extracts from both mice, catalase activities dwindled and disappeared in the same way and peroxidatic activities appeared if treated with SDS. But peroxidatic activity of acatalasemia liver extract was higher than that of normal liver extract. It seems that the liver catalase in acatalasemia mouse is more labile by SDS treatment than that in normal mouse, resulting in showing the conformational changes of catalase molecule in acatalasemia mouse liver.
Published Date
1975-06-30
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume87
Issue
issue5-6
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
543
End Page
548
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/87/5-6/87_5-6_543/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma