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ID 18258
Eprint ID
18258
FullText URL
Thumnail 76_201.pdf 1.37 MB
Title Alternative
Tissue Culture of C(3)H Mouse Mammary Carcinoma Part 1. Properties of the long-term cultured C(3)H mouse mammary carcinoma strain (Hei-Strain)
Author
Noda, Masahiko
Abstract
This paper presents some observations on the C(3)H-Hei strain cells, established from spontaneous mammary tumor of the C(3)H mouse. 1) The C(3)H-Hei strain has been propagated over 800 days ever since December 1961. 2) The most optimal medium consist of 50% bovine serum, 0,25% lactalbumine hydrolysate, 0,05% yeast extract and Earle's saline. 3) The primary culture and early subculture of this cell strain morphologically exhibited a number of cell sheets of epithelial-like cells, but suddenly at the 4th generation. they have transformed the morphological pattern from the epithelial-like cells to the fibroblast-like cells of a uniform size. 4) Tbe growth rate in cell population was about 8 folds in 6 days. 5) Chromosome number of the Hei-strain showed a higher pattern ranging from 64 to 72. 6) The tumor-inducing capacity of this strain was examined by subcutaneous back-inoculation into C3H, Zb and Cb mice. A certain portion of the mica had a total body irradiation (400r) before inoculation and it was found that 100% C(3)H mice challenged before injection had developed tumors, but Zb and Cb mice did not.
Published Date
1964-06-30
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume76
Issue
issue4-6
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
201
End Page
214
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/76/4-6/76_4-6_201/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma