ID 32202
JaLCDOI
FullText URL
Author
Ohmori, Toyonori
Takamoto, Kazuhiko
Ogata, Masana
Abstract

Some mechanisms to reduce methemoglobin (metHb) formation for the maintenance of normal oxygen transport have been proposed. To study the role of catalase (EC 1.11.1.6), metHb formation in the hemolysate of normal and Japanese acatalasemic human subjects were examined spectrophotometrically. Significantly increased level of metHb was induced by potassium ferrocyanide in the hemolysate of acatalasemic subject. The addition of catalase reduced the metHb formation, while 3-amino-1,2,4-triazole (AT), a specific inhibitor of catalase-H2O2 compound I, increased it. These results obtained from human subjects were well consistent with those from mice and suggested that catalase plays a role in protecting erythrocytes against metHb formation.

Keywords
methemoglobin
catalase
acatalasemia
potassium ferrocyanide
biological monitoring
Amo Type
Article
Published Date
1991-10
Publication Title
Acta Medica Okayama
Volume
volume45
Issue
issue5
Publisher
Okayama University Medical School
Start Page
321
End Page
324
ISSN
0386-300X
NCID
AA00508441
Content Type
Journal Article
language
英語
File Version
publisher
Refereed
True
PubMed ID
Web of Sience KeyUT