ID 32414
JaLCDOI
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Author
Kurosaki, Tomohiro
Tokuda, Masaaki
Hatase, Osamu
Abstract

2-Mercaptoethanol increases the optical density of assay solutions at wavelengths between 280 to 400 nm, and therefore interferes with the measurement of protein concentration by the microbiuret method. Protein concentration can be determined in the presence of 2-mercaptoethanol up to 6 mM by modification of the method as follows: after the precipitation of protein by trichloroacetic acid in the presence of deoxycholate, the precipitate is resolubilized with NaOH solution. Dithiothreitol interfered with the protein determinations could by made in the presence of 4 mM of dithiothreitol with the modified microbiuret method. This modified method is time-saving and more reliable than other methods for protein determination, such as Lowry's method, in the presence of sulfhydryl reagents.

Keywords
microbiuret method
sulfhydryl reagent
protein determination
Amo Type
Article
Published Date
1983-04
Publication Title
Acta Medica Okayama
Volume
volume37
Issue
issue2
Publisher
Okayama University Medical School
Start Page
125
End Page
129
ISSN
0386-300X
NCID
AA00508441
Content Type
Journal Article
language
英語
File Version
publisher
Refereed
True
PubMed ID
Web of Science KeyUT