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ID 34114
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Author
Sasabe, Michiko
Naito, Kana
Suenaga, Hiroko
Ikeda, Takako
Toyoda, Kazuhiro Kakenhi
Shiraishi, Tomonori Kakenhi
Abstract

The inhibition of elicitor-induced plant defense responses by the protein kinase inhibitors K252a and staurosporine indicates that defense responses require protein phosphorylation. We isolated a cDNA clone encoding Nicotiana tabacum lectin-like receptor protein kinase 1 ( NtlecRK1), an elicitor-responsive gene; in tobacco bright yellow ( BY-2) cells by a differential display method. NtlecRK forms a gene family with at least three members in tobacco. All three NtlecRK genes potentially encode the N-terminal legume lectin domain, transmembrane domain and C-terminal Ser/Thr-type protein kinase domain. Green fluorescent protein ( GFP) fusion showed that the NtlecRK1 protein was located on the plasma membrane. In addition, NtlecRK1 and 3 were responsive to INF1 elicitin and the bacterial elicitor harpin. These results indicate that NtlecRKs are membrane-located protein kinases that are induced during defense responses in BY-2 cells.

Keywords
defense response
elicitin
lectin
receptor-like kinase
tobacco BY-2
Note
Published with permission from the copyright holder.
This is published in DNA Sequence, 2007 Vol.18 Issue.2 pp.152-159
Publisher URL: http://dx.doi.org/10.1080/10425170601060905
Direct access to Thomson Web of Science record
(C)2008 by Informa plc.
Published Date
2007-01-01
Publication Title
DNA Sequence
Volume
volume18
Issue
issue2
Start Page
152
End Page
159
Content Type
Journal Article
language
英語
Refereed
True
DOI
Web of Sience KeyUT
Submission Path
microbiology_and_immunology/11