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ID 40611
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Title Alternative
Experimental studies on lung reperfusion injury with leukocyte-depleted blood
Author
Kawada, Shinsaku
Abstract
The histological appearance of reperfusion injury is pulmonary edema associated with leukocyte aggregation in the vessels. We hypothesized that leukocytes play an important role in reperfusion injury and that leukocyte depletion could prevent it. Eighteen white male rabbits were divided into 3 groups. After extirpation, the lungs in group 1 were not preserved, and were reperfused with whole blood that was taken from other white rabbits. In groups 2 and 3, after flushing with 100 ml of modified Collins' solution, the lungs were preserved in the same solution for 6 hours at 4°C. Lungs in group 2 were then reperfused with whole blood while lungs in group 3 were reperfused with leukocyte-depleted blood. We measured the water content of the lung to determine the degree of pulmonary damage, the malondialdehyde (MDA) levels in reperfused blood and lung tissue, the complement activity, and examined the histological findings. Group 2 showed severe pulmonary edema histologically, and a significantly higher water content and MDA level than the other groups. There was no significant difference in complement activity between the 3 groups. Leukocyte depletion was effective in reducing reperfusion injury of the lung.
Keywords
肺保存
再灌流障害
白血球除去
活性酸素
MIDA
肺水分量
Published Date
1990-04
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume102
Issue
issue3-4
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
309
End Page
318
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/102/3-4/102_3-4_309/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma