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Ye, Wenxiu Graduate School of Environmental and Life Science, Okayama University
Ando, Eigo Graduate School of Science, Nagoya University
Rhaman, Mohammad Saidur Graduate School of Environmental and Life Science, Okayama University
Tahjib-Ul-Arif, Md Graduate School of Environmental and Life Science, Okayama University
Okuma, Eiji Graduate School of Environmental and Life Science, Okayama University
Nakamura, Yoshimasa Graduate School of Environmental and Life Science, Okayama University ORCID Kaken ID publons
Kinoshita, Toshinori Institute of Transformative Bio-Molecule, Nagoya University
The glucosinolate-myrosinase system is a well-known defense system that has been shown to induce stomatal closure in Brassicales. Isothiocyanates are highly reactive hydrolysates of glucosinolates, and an isothiocyanate, allyl isothiocyanate (AITC), induces stomatal closure accompanied by elevation of free cytosolic Ca2+ concentration ([Ca2+](cyt)) in Arabidopsis. It remains unknown whether AITC inhibits light-induced stomatal opening. This study investigated the role of Ca2+ in AITC-induced stomatal closure and inhibition of light-induced stomatal opening. AITC induced stomatal closure and inhibited light-induced stomatal opening in a dose-dependent manner. A Ca2+ channel inhibitor, La3+, a Ca(2+)chelator, EGTA, and an inhibitor of Ca2+ release from internal stores, nicotinamide, inhibited AITC-induced [Ca2+](cyt) elevation and stomatal closure, but did not affect inhibition of light-induced stomatal opening. AITC activated non-selective Ca2+-permeable cation channels and inhibited inward-rectifying K+ (K-in(+)) channels in a Ca2+-independent manner. AITC also inhibited stomatal opening induced by fusicoccin, a plasma membrane H+-ATPase activator, but had no significant effect on fusicoccin-induced phosphorylation of the penultimate threonine of H+-ATPase. Taken together, these results suggest that AITC induces Ca2+ influx and Ca2+ release to elevate [Ca2+](cyt), which is essential for AITC-induced stomatal closure but not for inhibition of K-in(+) channels and light-induced stomatal opening.
Journal of Experimental Botany
Oxford University Press
© The Author(s) 2020.
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