ID 60776
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Author
Tsuruta, Takeshi Laboratory of Animal Nutrition, Graduate School of Environmental and Life Science, Okayama University ORCID Kaken ID researchmap
Katsumata, Emiko Laboratory of Animal Nutrition, Graduate School of Environmental and Life Science, Okayama University
Mizote, Akiko Hayashibara Co., Ltd.
Jian, Hou Jian Laboratory of Animal Nutrition, Graduate School of Environmental and Life Science, Okayama University
Muhomah, Teresia Aluoch Laboratory of Animal Nutrition, Graduate School of Environmental and Life Science, Okayama University
Nishino, Naoki Laboratory of Animal Nutrition, Graduate School of Environmental and Life Science, Okayama University ORCID Kaken ID publons researchmap
Abstract
Cyclic nigerosylnigerose (CNN) is a cyclic oligosaccharide. Oral administration of CNN promotes immunoglobulin A (IgA) secretion in the gut. IgA is a major antibody secreted into the gut and plays a crucial role in suppressing gut inflammation due to commensal gut microbiota. To investigate the effect of administration of CNN to promote IgA secretion on gut inflammation, experimental colitis was induced with dextran sulfate sodium (DSS) in Balb/c mice after 6 weeks of CNN pre-feeding. The severity of colitis was evaluated based on a disease activity index (DAI), the gene expression of inflammatory cytokines, and a histological examination. The CNN-treated mice with DSS-induced colitis (CNN-DSS group) showed significantly lower DAI scores and mRNA levels of interleukin-1 compared with the CNN-untreated mice with DSS-induced colitis (DSS group). Histological examination of the colon revealed that the pathological score was significantly lower in the CNN-DSS group compared with the DSS group due to the reduced infiltration of immune cells. The number of goblet cells was significantly higher in the CNN-DSS group compared with the DSS group. The IgA concentration and the ratio of microbiota coated with IgA were evaluated in the cecal content. Although there was no difference in the IgA concentration among groups, a higher proportion of cecal microbiota were coated with IgA in the CNN-DSS group compared with that in the DSS group. These results suggest that CNN might preserve goblet cells in the colon and promote IgA coating of gut microbiota, which synergistically ameliorate gut inflammation in mice with DSS-induced colitis.
Keywords
oligosaccharide
DSS-induced colitis
goblet cell
gut microbiota
immunoglobulin A
Published Date
2020
Publication Title
Bioscience of Microbiota, Food and Health
Volume
volume39
Issue
issue3
Publisher
BMFH Press
Start Page
188
End Page
196
ISSN
2186-3342
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
Copyright Holders
© 2020 by BMFH Press
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Related Url
isVersionOf https://doi.org/10.12938/bmfh.2020-012
License
https://creativecommons.org/licenses/by-nc-nd/4.0/