Published by Misasa Medical Center, Okayama University Medical School
Published by Misasa Medical Center, Okayama University Medical School

<Formerly known as>
岡大三朝分院研究報告 (63号-72号) 環境病態研報告 (57号-62号)
岡山大学温泉研究所報告 (5号-56号) 放射能泉研究所報告 (1号-4号)

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ラット腹腔肥満細胞に関する研究 第1報 : 肥満細胞のpurification について

Tanizaki, Yoshiro
Tanaka, Juntaro
Komagoe, Haruki
Morinaga, Hiroshi
Kishimoto, Takumi
Kimura, Ikuro
Townley Robert G.
Abstract
To get a high recovery rate of mast cells from the rat peritoneal cavity, a new modified method was discussed using BSA density gradient. Three procedures, peritoneal lavage, washing of peritoneal cells and purification by BSA, were tested in this study, and the numbers of total cells and mast cells in each procedure were calculated. The recovery rate was the highest in BSA density gradient purification of the cells washed once with low speed centrifugation. The recovery rate was 73.7% and the number of mast cells taken from one rat was approximately 1.5×10(6). The purity of the cells with this procedure was 95.0% (range from 93.0 to 96.0%).
Note
原著論文 (Original Papers)
ISSN
0369-7142
NCID
AN00032853