Scientific Reports of the Faculty of Agriculture, Okayama University
Published by the Faculty of Agriculture, Okayama University
ONLINE ISSN : 2186-7755

マツノザイセンチュウ,Bursaphelenchus xylophilusに随伴する松萎凋性細菌の単離とその毒性代謝物質

Kawazu, Kazuyoshi
Yamasita, Hideaki
Kobayasi, Akio
Abstract
Based on the observation that ray parenchyma cell death took place prior to nematode population increase in pine wood,the authors suspected that any microorganisms carried by pathogenic nematodes would be involvedin the pathogenic process.The authors screened pathogenic nematode-accompanying microbes for their toxicity against cultured cells of Pinus thunbergiis and isolated and identified 3 toxic strains,Bacillus cereus HY-3,B.subtilis HY-16,and Bmegaterium HY-17 , whose toxic products were identified as phenylacertic acid.Inoculation of pine seedlings with the toxic bacterium alone did not cause the seedling to wilt , but inoculation of pine seedlings with the toxic bacterium carried by weakly pathogenic nematodes wilted the seedling as much as strongly pathogenic nematodes . These results suggested that phenyllacetic acid-producing bacteria could invade pine trees by accompanying pine wood nematodes , and produce phenylacetic acid , a toxic metabolite , in the wood.
Keywords
pathogenic bacteria
phenylacetic acid producer
nematode-accompanying bacteria
fluorescence microscopic analysis
pine wilt disease
ISSN
0474-0254
NCID
AN00033029