Scientific Reports of the Faculty of Agriculture, Okayama University
Published by the Faculty of Agriculture, Okayama University
ONLINE ISSN : 2186-7755

Cyclo (Gly-Tyr)分解酵素の合成高分子配糖体による安定化

Abe, Yasuteru
Kawazu, Kazuyoshi
Abstract
An enrichment technique led us to isolate 13 cyclo (Gly-Tyr)-assimilating bacteria, which had higher cyclo (Gly-Tyr)-hydrolyzing activity that the coryneform rod bacterium strain T-1-3-Y,previously isolated from soil. The cell-free extract of the strain Ol-L-2 prepared by ultrasonication in a buffer containing bovine serum albumin had cyclo (Gly-Tyr)-hydrolyzing activity while that of the strain T-1-3-Y showed no activity. GEMA (Glucosyloxyethyl methacrylate) polymer was found to be a more effective stabilizer for solubilizing cyclo (Gly-Tyr) hydrolase than bovine serum albumin.
Keywords
diketopiperazine
cyclic dipeptide
hydrolysis
protease
ISSN
0474-0254
NCID
AN00033029