Scientific Reports of the Faculty of Agriculture, Okayama University
Published by the Faculty of Agriculture, Okayama University
ONLINE ISSN : 2186-7755

長波長紫外光照射による酵母 Rhodotorula minuta 細胞膜エルゴステロールの分解

Nakauma, Makoto
Arami, Shin-ichiro
Hada, Megumi
Nakagawa, Ichiro
Tada, Mikiro
Abstract
In search for primary photochemical reaction related to photoinduced carotenogenesis and photoinactivivation in yeast Rhodotorula minuta, the influence of near-UV radiation (300-400 nm) on ergosterol in the plasma membrane of the yeast. Irradiation by near-UV of intact cells and the plasma membrane preparation from the yeast caused changes in their absorption spectra. For the plasma membrane, difference spectra measured after irradiation of near-UV suggested the decomposition of ergosterol and the suggestion was proved by HPLC analysis. Experiments in model systems revealed that ergosterol itself was the photoreceptor in the photochemical reaction, and that there was not any other photoreceptor or photosensitizer which was related to photochemical reaction of ergosterol decomposition in the plasma membrane.
Keywords
Rhodotorula minuta
near-UV
ergosterol
absorbance change
ISSN
0474-0254
NCID
AN00033029