Scientific Reports of the Faculty of Agriculture, Okayama University
Published by the Faculty of Agriculture, Okayama University
ONLINE ISSN : 2186-7755

Structural Analysis of Free N-Glycans in Bamboo (Phyllostachys heterocycla) Shoots

Kimura, Yoshinobu Kakenhi
Ueyama, Tatsuya
Abstract
Free N-glycans, the high-mannose-type and the plant complex-type, have been found in bamboo shoots. These free N-glycans were coupled with 2-aminopyridine and purified by gel filtration, Con A-Sepharose affinity chromatography, reversed-phase HPLC, and size-fractionation HPLC. The structures of these pyridylaminated free N-glycans were identified by two-dimensional sugar chain mapping, exomannosidase digestions, and ion-spray tandem mass spectrometry. The structual analyses showed that the various free high-mannose type sugar chains having one GlcNAc(Man8-5GlcNAc1) and free xylose/fucose containing sugar chains having the chitobiose segment occur in the developing bamboo shoots, suggesting that an endo-β-N-acetylglucosaminidase should produce the former structures, and a peptide: N-glycanase should produce the latter structures.
Keywords
free N-glycan
plant N-glycan
endo-β-N-acetylglucosaminidase
peptide:N-glycanase
Phyllostachys heterocycla
ISSN
0474-0254
NCID
AN00033029