Scientific Reports of the Faculty of Agriculture, Okayama University
Published by the Faculty of Agriculture, Okayama University
ONLINE ISSN : 2186-7755

Efficiet Inactivation of Pepper Mild Mottle Virus(PMMoV) in Harvested Seeds of Green Pepper(Capsicum annuum L.) Assessed by a Reverse Transcription and Polymerase Chain Reaction (RT-PCR)-based Amplification

Hikichi, Yasufumi
Takeuchi, Shigeharu
Okumura, Ako
Nasu, Yoshiko
Okuno, Tetsuro
Suzuki, Kazumi
Abstract
A method for efficient inactivation Papper mild mottle virus (PMMoV) in harvested seeds of green pepper was examined based on the infectivity on the leaves of Nicotina glutinosa L.(a local host) and a reverse transcription and polymerase chain reaction (RT-PCR)-based amplification of the viral RNA. The seed homogenates from PMMoV-infected plants produced a large number of necrotic local lesion in N.glutinosa, but soaking the seeds in 10% (w/v) tri-sodium phosphate (Na3PO4) for 20 min or dry sterilization (70C,3h) effectivity eliminated the abundance of PMMoV. However, no necrotic lesion on N.glutinosa or the RT-PCR-basaed amplification was observed with seeds that has been disinfected by Na3PO4 in combination with dry sterilization.
Keywords
Capsicum annuum L.
pepper mild mottle virus (PMMoV)
RT-PCR
seed disinfection
seed transmission
ISSN
0474-0254
NCID
AN00033029