このエントリーをはてなブックマークに追加
ID 32975
FullText URL
Author
Taguchi, Fumiko
Takeuchi, Kasumi
Katoh, Etsuko
Murata, Katsuyoshi
Suzuki, Tomoko
Marutani, Mizuri
Kawasaki, Takayuki
Eguchi, Minako
Katoh, Shizue
kaku, Hanae
Yasuda, Chihiro
Abstract
A glycosylation island is a genetic region required for glycosylation. The glycosylation island of flagellin in Pseudomonas syringae pv. tabaci 6605 consists of three orfs: orf1, orf2 and orf3. Orf1 and orf2 encode putative glycosyltransferases, and their deletion mutants, Delta orf1 and Delta orf2, exhibit deficient flagellin glycosylation or produce partially glycosylated flagellin respectively. Digestion of glycosylated flagellin from wild-type bacteria and non-glycosylated flagellin from Delta orf1 mutant using aspartic N-peptidase and subsequent HPLC analysis revealed candidate glycosylated amino acids. By generation of site-directed Ser/Ala-substituted mutants, all glycosylated amino acid residues were identified at positions 143, 164, 176, 183, 193 and 201. Matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry (MS) analysis revealed that each glycan was about 540 Da. While all glycosylation-defective mutants retained swimming ability, swarming ability was reduced in the Delta orf1, Delta orf2 and Ser/Ala-substituted mutants. All glycosylation mutants were also found to be impaired in the ability to adhere to a polystyrene surface and in the ability to cause disease in tobacco. Based on the predicted tertiary structure of flagellin, S176 and S183 are expected to be located on most external surface of the flagellum. Thus the effect of Ala-substitution of these serines is stronger than that of other serines. These results suggest that glycosylation of flagellin in P. syringae pv. tabaci 6605 is required for bacterial virulence. It is also possible that glycosylation of flagellin may mask elicitor function of flagellin molecule.
Keywords
Gram-Negative bacteria
Posttranslational modification
Protein Glycosylation
Perception
Aeruginosa
Cells
Specificity
Expression
Plasmids
Pathways
Note
Digital Object Identifer:10.1111/j.1462-5822.2005.00674.x
Published with permission from the copyright holder. This is the author's copy, as published in Cellular Microbiology, June 2006, Volume 8, Issue 6, Pages 923-938. Publisher URL:http://dx.doi.org/10.1111/j.1462-5822.2005.00674.x
Direct access to Thomson Web of Science record
Copyright © 2006 The Authors Journal compilation © 2006 Blackwell Publishing Ltd.
Published Date
2006-6
Publication Title
Cellular Microbiology
Volume
volume8
Issue
issue6
Publisher
Blackwell Publishing
Start Page
923
End Page
938
ISSN
1462-5814
NCID
AA1136678X
Content Type
Journal Article
language
英語
Copyright Holders
The Authors, Journal compilation : Blackwell Publishing Ltd
File Version
author
Refereed
True
DOI
PubMed ID
Web of Sience KeyUT
Submission Path
biology_general/24