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Author
Marutani, Mizuri
Taguchi, Fumiko
Ogawa, Yujiro
Hossain, Mijan Md.
Yoshishige, Inagaki
Abstract

Pseudomonas syringae pv. tabaci 6605 causes wildfire disease on host tobacco plants. To investigate the regulatory mechanism of the expression of virulence, Gac two-Component system-defective mutants, Delta gacA and Delta gacS, and a double mutant, Delta gacA Delta gacS, were generated. These mutants produced smaller amounts of N-acyl homoserine lactones required for quorum sensing, had lost swarming motility, and had reduced expression of virulence-related hrp genes and the algT gene required for exopolysaccharide production. The ability of the mutants to cause disease symptoms in their host tobacco plant was remarkably reduced, while they retained the ability to induce hypersensitive reaction (HR) in the nonhost plants. These results indicated that the Gac two-component system of P. syringae pv. tabaci 6605 is indispensable for virulence on the host plant, but not for HR induction in the nonhost plants.

Keywords
GacA
GacS
Pseudomonas syringae pv. tabaci
quorum sensing
two-component system
Note
Published with permission from the copyright holder.
This is published in Molecular Genetics and Genomics, 2008 Vol.279 Issue.4 pp.313-322
Publisher URL: http://dx.doi.org/10.1007/s00438-007-0309-y
Direct access to Thomson Web of Science record
(C)2007 Springer-Verlag
Published Date
2008-04
Publication Title
Molecular Genetics and Genomics
Volume
volume279
Issue
issue4
Publisher
Springer
Start Page
313
End Page
322
Content Type
Journal Article
language
英語
Copyright Holders
Springer-Verlag
Refereed
True
DOI
Web of Sience KeyUT
Submission Path
biochemistry/17