岡山医学会 Acta Medica Okayama 0030-1558 71 1 1959 焦性葡萄酸及びα-ケトグルタール酸に関する臨床的研究 第Ⅲ編 グルタミン酸ソーダを負荷した場合の血液,腰椎髄液,脳室髄液中の焦性葡萄酸及びα-ケトグルタール酸の消長について 67 72 EN Kosai Uenaga In order to study the mechanism of glutamic acids involved in the metabolisms of pyruvate and α-ketoglutaric acid in blood, spinal fluid of lumbar region, and ventricle fluid, the author gave oral administration of dailydosage of 15 g sodium glutamate for five days to schizophrenic patients without any apparent disorders of organs, and determined the amounts of both acids in blood when the patients were at rest both before and after the sodium glutamate administration. Then after lobotomy, blood, spinal fluid of lumbar regions and ventricle Fluid were drawn and the contents of both acids in these fluids were determined in the same manner as mentioned above. As the results: 1. In the estimations of both acids in the blood drawn both before and after the administration of sodium glutamate to the patient at rest, no fixed tendency suggestive of the effect of this drug could be recognized. 2. In the cases where lobotomy had been performed after administration of sodium glutamate and the contents of both acids were determined, and the following results were obtained: 1. The amount of pyruvate in blood=1.00±0.06 mg/dl; the amount of α-ketoglutarate in blood=0.41±0.06 mg/dl; the amount of pyruvate in spinal fluid of lumbar region=0.69±0.02 mg/dl; the amount of α-ketoglutaric acid in spinal fluid of lumbar region=0.35±0.03 mg/dl; pyruvate in ventricle fluid=0.80±0.02 mg/dl; and α-ketoglutaric acid in ventricle fluid=1.83±0.13 mg/dl. On comparing each of these values with respective values of the control reported in Part 1, both amounts of pyruvate and α-ketoglutaric acid in blood and spinal fluid of lumbar region show a decreasing tendency. This phenomenon seems to be due to the tranquilizing effect of sodium glutamate on the central nervous system or to improved liver function due to this drug. On the other hand, in the case of the ventricle fluid the pyruvate content was less than that in the control, and the content of α-ketoglutaric acid was somewhat increased. This fact seems to indicate that glutamic acid is involved in the metabolism of ketoacid in the cerebral fluid and is also associated with the glutamic acid metabolism in the brain, and also it is suggestive of some significance relative to the supply of glutamic acids in the brain. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 71 1 1959 焦性葡萄酸及びα-ケトグルタール酸に関する臨床的研究 第Ⅱ編 クロールプロマジン及びイソミタールを負荷した場合の血液・腰椎髄液・脳室髄液中の焦性葡萄酸及びα-ケトグルタール酸の消長について 59 66 EN Kosai Uenaga With the purpose to study the effect of chlorpromazine on body fluids in vivo, especially the changes in the amounts of pyruvate and α-ketoglutaric acid in ventricle fluid, spinal fluid of lumbar region and blood, the author estimated according to Shimazono-Shimizu method the amounts of pyruvate and α-ketoglutaric acid in blood and spinal fluids obtained from schizophrenic patients at rest or after adminstration of the optimum dosage of chlorpromazine (C. P.) or iaomytal (Iso) and given lobotomy. The results are as follows: 1. No marked difference between the amount of pyruvate or α-ketoglutaraic acid in the venous blood obtained from the persons at rest with empty stomach and the same obtained after the administration of 200 mg C. P for 10 days or intramascular injection of 50 mg C. P. 2. In the estimation of two acid amounts in ventricle fluid, spinal fluid of lumbar regions and elbow venous blood obtained at about the same time after intramascular injection of 100 mg C. P, it has been found to be in the order of the amount of pyruvate in blood > the same in ventricle fluid > that in spinal fluid of lumbar region. The amount of α-ketoglutaric acid proves to be in the order of that in ventricle fluid > that in blood > that in spinal fluid of lumbar region. Each of these values shows a slightly decreasing tendency as compared with those in the control to which no drug has been administered. 3. After the intravenous administration of 0.5 g Isomytal (Iso) the amounts of both acids distributed at various sites have been estimated in the same manner as above. In this instance the amounts of pyruvate and α-ketoglutaric acid distributed at different sites show about the same tendency as observed in the control as well as in the cases given C. P. However, when these values are compared with those given C, P. and those at each site, the values of both acids in blood and in spinal fluid at lumbar region show a decreasing teudency as compared with the control, but in ventricle fluid the amount of α-ketoglutaric acid is slightly increased. In other words, it appears that the changes in the amount of α-ketoglutaric acid in cerbral fluid behave differently from those in the case given C. P. Moreover, this phenomenon seems to be due to the difference in the mechanism of C. P. and Iso. acting on the brain. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 71 1 1959 焦性葡萄酸及びα-ケトグルタール酸に関する臨床的研究 第Ｉ編 血液・腰椎髄液・脳室髄液・蜘網膜下髄液中の焦性葡萄酸及びα-ケトグルタール酸について 51 58 EN Kosai Uenaga 1)著者は正常人並びに分裂病者の空腹安静時における,血中焦ブ並びにα-ケトグ量を測定して夫々次の様な結果を得た.イ)正常人 血中焦ブ量=0.84±0.05 mg/dl 血中α-ケトグ量=0.45±0.017 mg/dl ロ)分裂病者血中焦ブ量=0.79±0.039 mg/dl 血中α-ケトグ量=0.42±0.03 mg/dl 即ち分裂病者の血中両ケト酸平均値は,正常人のそれと略等しく,両者の間に有意の差を認めなかつた.2)器質的疾患を認めない分裂病者9例に脳手術(ロボトミー)を行い,略同時に夫々肘静脈血,腰椎髄液,脳室髄液を採取し,これらの焦ブ量,α-ケトグ量を測定し次の如き結果を得た.イ):血中焦ブ量=1.29±0.11 mg/dl 血中α-ケトグ量=0.59±0.10mg/dl ロ)腰椎髄液中焦ブ量=0.80±0.06 mg/dl: 腰椎髄液中α-ケトグ量=0.48±0.09 mg/dlハ):脳室髄液中焦ブ量=0.91±0.11 mg/dl :脳室髄液中α-ケトグ量=1.47±0.17 mg/dl 即ら焦ブ量濃度は血液,脳室髄液,腰椎髄液の順であり,α-ケトグ量は脳室髄液に尤も多く,次いで血液,腰椎髄液の順を示した.3)種々の条件の下で,肘静脈血,腰椎髄液,脳室髄液,脳表より採取した蜘網膜下髄液について両ケト酸を測定した所,蜘網膜下髄液中の両ケト酸は共に,腰椎髄液中のそれらと略等しい値を示した. No potential conflict of interest relevant to this article was reported.