Author Bekku, Yoko| Ninomiya, Yoshifumi| Oohashi, Toshitaka|
Published Date 2012-04-01
Publication Title 岡山医学会雑誌
Volume volume124
Issue issue1
Content Type Journal Article
Author Ninomiya, Yoshifumi|
Published Date 1997-10
Publication Title 岡山実験動物研究会報
Volume volume14
Content Type Others
Author Obika, Masanari| Ogawa, Hiroko| Takahashi, Katsuyuki| Li, Jiayi| Hatipoglu, Omer Faruk| Cilek, Mehmet Zeynel| Miyoshi, Toru| Inagaki, Junko| Ohtsuki, Takashi| Kusachi, Shozo| Ninomiya, Yoshifumi| Hirohata, Satoshi|
Published Date 2012-10
Publication Title Cancer Science
Volume volume103
Issue issue10
Content Type Journal Article
JaLCDOI 10.18926/AMO/31831
FullText URL fulltext.pdf
Author Hatipoglu, Omer Faruk| Hirohata, Satoshi| Yaykasli, Kursat Oguz| Cilek, Mehmet Zeynel| Demircan, Kadir| Shinohata, Ryoko| Yonezawa, Tomoko| Oohashi, Toshitaka| Kusachi, Shozo| Ninomiya, Yoshifumi|
Abstract <p>ADAMTS1 (a disintegrin and metalloproteinase with thrombospondin motifs 1) is an inflammatory-induced gene. We have previously reported that ADAMTS1 was strongly but transiently expressed in the infarcted heart. In this study, we investigated whether a 3'-untranslated region (UTR) affects the mRNA stability of this gene. When stimulated with tissue necrosis factor (TNF)-alpha, the expression level of ADAMTS1 mRNA rapidly increased, but the induction of ADAMTS1 mRNA peaked at 6h after stimulation, after which the expression levels of ADAMTS1 mRNA decreased. The 3'-UTR ADAMTS1 mRNA contains multiple adenine and uridine-rich elements, suggesting that the 3'-UTR may regulate gene stability. The addition of actinomycin D, an RNA synthesis inhibitor, demonstrated the decay of induced ADAMTS1 mRNA by TNF-alpha. Furthermore, a region containing multiple AUUUA motifs within the ADAMTS1 3'-UTR destabilized transfected Enhanced Green Fluorescence Protein (EGFP) mRNA expression. These results demonstrated that the ADAMTS1 3'-UTR may regulate the expression of ADAMTS1 mRNA.</p>
Keywords ADAMTS1 gene regulation metalloproteinase
Amo Type Original Article
Published Date 2009-04
Publication Title Acta Medica Okayama
Volume volume63
Issue issue2
Publisher Okayama University Medical School
Start Page 79
End Page 85
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 19404339
Web of Sience KeyUT 000265457600002
JaLCDOI 10.18926/AMO/30509
FullText URL fulltext.pdf
Author Yanai, Hiroyuki| Yoshino, Tadashi| Takahashi, Kiyoshi| Ninomiya, Yoshifumi| Akagi, Tadaatsu|
Abstract <p>Circulating hepatitis C virus (HCV) particles can be fractionated by means of differential flotation centrifugation. It is reported that in the bottom fraction HCV is in the form immune complexes, whereas in the top, it is free of antibodies. We evaluated the significance of circulating complex and free HCV in chronic hepatitis C, and assessed the relationship in terms of the response to interferon (IFN) therapy. We examined sera before, just after, and 1 year after administering IFN to 18 patients with chronic hepatitis C, 10 of whom responded (group CR), and 8 did not (group NR). The amounts of virus were similar between both groups before therapy. After differential flotation centrifugation with 1.063 g/ml of NaCl, the top and bottom fractions were assayed for HCV RNA. Before therapy, HCV RNA was detected in the top fraction in 1 of 10 in group CR, and in 6 of 8 in group NR (P &#60; 0.05, chi-square test). HCV RNA was positive in the bottom fraction of all samples. In a follow-up study of group NR, HCV RNA was detected in the top fraction in 3 of 8 just after IFN therapy, and in 7 of 8 after 1 year. This study suggests that the presence of HCV in the top fraction can predict a poor response to IFN therapy.</p>
Keywords IL-2R ??chain phorbol ester monocyte differentiation protein kinase
Amo Type Article
Published Date 1996-06
Publication Title Acta Medica Okayama
Volume volume50
Issue issue3
Publisher Okayama University Medical School
Start Page 145
End Page 150
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 8805853
Web of Sience KeyUT A1996UU60400005
Author Fleischmajer, Raul| Utani, Atsushi| Douglas MacDonald II, E.| Perlish, Jerome S| Pan, Te-Cheng| Chu, Mon-Li| Nomizu, Motoyoshi| Ninomiya, Yoshifumi| Yamada, Yoshihiko|
Published Date 1998-7
Publication Title Journal of Cell Science
Volume volume111
Issue issue14
Content Type Journal Article
JaLCDOI 10.18926/AMO/31728
FullText URL fulltext.pdf
Author Nomoto, Hiroyuki| Oohashi, Toshitaka| Hirakawa, Satoshi| Ueki, Yasuyoshi| Ohtsuki, Hiroshi| Ninomiya, Yoshifumi|
Abstract <p>We herein determined by fluorescence in situ hybridization the chromosomal localization of 2 human genes, BRAL1 and BCAN, both of which belong to the link-module superfamily, i.e. to the same band of chromosome 1q21-23. Further analysis of the genomic organization of BRAL1 and BCAN revealed that the BRAL1 gene was located 20-kb upstream of the BCAN start site. We isolated a polymorphic dinucleotide (CA) repeat sequence from a genomic clone containing the BCAN gene. High heterozygosity (0.79) makes this polymorphism a useful marker in the study of genetic disorders. Knowledge of the structure of the genes and the marker provides essential information for further analysis of the gene locus at chromosome 1q21-23.</p>
Keywords BRAL1 BCAN FISH schizophrenia polymorphic marker
Amo Type Article
Published Date 2002-02
Publication Title Acta Medica Okayama
Volume volume56
Issue issue1
Publisher Okayama University Medical School
Start Page 25
End Page 29
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 11873941
Web of Sience KeyUT 000174031300005
JaLCDOI 10.18926/AMO/32300
FullText URL fulltext.pdf
Author Nakamura, Koki| Irie, Hiroyuki| Fujisawa, Emi| Yoshioka, Hidekatsu| Ninomiya, Yoshifumi| Sakuma, Isao| Sano, Shunji|
Abstract <p>While heat shock protein (HSP) 72 is known as a stress protein, there have been no reports of HSP 72 expression in patients who have undergone surgery for congenital heart disease. Fourteen patients (7 males and 7 females) who had undergone surgery for congenital heart disease were studied. The ages of the patients ranged from 2 months to 43 years old (mean 6.5 +/- 10.8 years old; median 3.0 years old). The diagnoses were Tetralogy of Fallot in seven, pulmonary atresia with ventricular septal defect (VSD) in three, complex anomalies in three, and VSD in one patient. Histological study and HSP analysis using Western blots and immunostaining with anti-HSP 72 monoclonal antibody were performed for right ventricular muscle samples resected during the surgery. The histological findings showed hypertrophic changes of ventricular cardiomyocytes in all samples studied. Western blots detected HSP 72 expression of various degrees in all specimens. Immunostaining using monoclonal antibody against HSP 72 showed that the protein was present in the nuclei and cytoplasm of cardiomyocytes. In conclusion, although it is difficult to determine the cause of the &#34;stress&#34; that triggers HSP 72 expression in cardiomyocytes, low O2 saturation and pressure overload might act as a &#34;stress&#34;, and the only common factor that induced HSP 72 in every sample was hypertrophy.</p>
Keywords heat shock protein 72 (HSP 72) human heart congentional cardiac surgery hypertrophy
Amo Type Article
Published Date 2000-06
Publication Title Acta Medica Okayama
Volume volume54
Issue issue3
Publisher Okayama University Medical School
Start Page 103
End Page 109
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 10925734
Web of Sience KeyUT 000087965700002
JaLCDOI 10.18926/AMO/53521
FullText URL 69_3_145.pdf
Author Ishii, Hiroko| Kamikawa, Shigeshi| Hirohata, Satoshi| Mizutani, Akifumi| Abe, Koji| Seno, Masaharu| Oohashi, Toshitaka| Ninomiya, Yoshifumi|
Abstract Eosinophil cationic protein (ECP) is well known as a cationic protein contained in the basic granules of activated eosinophils. Recent studies have reported that ECP exhibits novel activities on various types of cells, including rat neonatal cardiomyocytes. Here we evaluated the effects of ECP on rat cardiac myoblast H9c2 cells. Our results showed that ECP enhanced the survival of the cells, in part by promoting the ERK and Akt/GSK-3β signaling pathways. ECP attenuated the cytotoxic effects of H2O2 on H9c2 cells as well as the production of reactive oxygen species, the number of apoptotic cells and caspase 3/7 activity in the cells. In conclusion, ECP activated the ERK and Akt/GSK-3β pathways, resulting in anti-oxidative effects on H9c2 cells that attenuated apoptosis.
Keywords ECP reactive oxygen species Akt ERK
Amo Type Original Article
Published Date 2015-06
Publication Title Acta Medica Okayama
Volume volume69
Issue issue3
Publisher Okayama University Medical School
Start Page 145
End Page 153
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
Copyright Holders CopyrightⒸ 2015 by Okayama University Medical School
File Version publisher
Refereed True
PubMed ID 26101190
Web of Sience KeyUT 000356903000003
Author Momota, Ryusuke| Naito, Ichiro| Ninomiya, Yoshifumi| Ohtsuka, Aiji|
Published Date 2011-05
Publication Title Matrix Biology
Volume volume30
Issue issue4
Content Type Journal Article
Author Pöschl, Ernst| Schlötzer-Schrehardt, Ursula| Brachvogel, Bent| Saito, Kenji| Ninomiya, Yoshifumi| Mayer, Ulrike|
Published Date 2004-04
Publication Title Development
Volume volume131
Issue issue7
Content Type Journal Article
Author Matsuura, Hiroko| Murakami, Takashi| Hina, Kazuyoshi| Yamamoto, Keizo| Kawamura, Hiroshi| Sogo, Taiji| Shinohata, Ryoko| Usui, Shinichi| Ninomiya, Yoshifumi| Kusachi, Shozo|
Published Date 2008-02
Publication Title Clinical Biochemistry
Volume volume41
Issue issue3
Content Type Journal Article
Author Demircan, Kadir| Hirohata, Satoshi| Nishida, Keiichiro| Hatipoglu, Omer F.| Oohashi, Toshitaka| Yonezawa, Tomoko| Apte, Suneel S.| Ninomiya, Yoshifumi|
Published Date 2005-5
Publication Title Arthritis & Rheumatism
Volume volume52
Issue issue5
Content Type Journal Article