このエントリーをはてなブックマークに追加
ID 19309
Eprint ID
19309
FullText URL
Thumnail 71_1529.pdf 1.52 MB
Title Alternative
Fundamental and Clinical Studies on Peripheral-Leucocyte Culture Part 1. Methodology and Cell multiplication in the Peripheral-Leucocyte Culture of Normal Persons
Author
Sogawa, Tamotsu
Abstract
With the purpose to maintain the cell function in a good condition for the peripheral-leucocyte culture the author compared various methods of tissue culture, and then investigated the tissue growth of peripheral leucocytes of normal persons in culture. The results are as follows: 1. For the preservation of blood to be cultured the use of the container whose inner surface coated with silicon is the best way to maintain the cell function as compared with the conventional method. 2. The expansion of growth area surrounding the original peripheral leucocyte mass in normal persons ceases after 6 hours' culture, and with the lapse of time the central zone of the growth area becomes less dense due to the emigration of wandering cells towards the peripheral zone, creating a space and thus this presents a corona-like appearance specific to this type of growth. 3. As for cells wandering out, the majority of them are neutrophils at the early stage of culture,and gradually numbers of eosinophils and lymphocytes increase; and after 12 hours' culture monocytes make their appearance. 4. As regards the distribution of cells in the growth area after 24 hours' culture, the peripheral zone is occupied mainly by neutrophils, and eosinopbils are scattered there, too; and the intermediate zone is chiefly occupied by lymphocytes and a few monocytes, but the central part is occupied by many monocytes.
Published Date
1959-03-15
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume71
Issue
issue3-2
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
1529
End Page
1535
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/71/3-2/71_3-2_1529/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma