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ID 19312
Eprint ID
19312
FullText URL
Thumnail 71_1537.pdf 456 KB
Title Alternative
Fundamental and Clinical Studies on Peripheral-Leucocyte Culture Part 2. The Function of Neutrophils in the Peripheral-Leucocyte Culture of Normal Persons
Author
Sogawa, Tamotsu
Abstract
In order to observe the cell function of peripheral leucocytes of normal persons by means of tissue culture, the author studied the wandering velocity, carbon-particle phagocytosis and neutral-red vital staining of cells from various angles; and arrived at the following conclusions: 1. Neutrophils begin to wander about actively already after 3 hours' culture, and after 6 hours' their behavior becomes still more vigorous, showing the maximum wandering velocity at this stage; but thereafter it gradually decreases, and after 24 hours' the wandering becomes very sluggish. 2. In pursuing the carbon-particle phagocytosis of various leucocytes, neutrophils and monocytes reveal a marked phagocytosis; especially the phagocytosis of monocytes is highest, followed by that of neutrophils; but no phagocytosis can be recognized in eosinophils, basophilocytes, and lymphocytes. 3. The average phagocytic capacity of neutrophils after 6 hours' culture reaches its maximum, but thereafter it declines very slowly. 4. As for the degree of stain to neutral-red vital staining in various leucocytes, at first a few granules in neutrophils and monocytes are stained lightly, but with the laspe of time numbers of these granules gradually increase and these stained granules. swelling up by confluence, present a peculiar arrangement in a form of rosette. In eosinophils and lymphocytes all granules are stained light red, and in basophilocytes these are stained deep red. All these granules are stained almost simultaneously, but the confluence of stained granules are not so marked. 5. The neutral-red vital staining of neutrophils is quite high already at the early stage of culture, and reaching the maximum after 3 hours' culture, thereafter it gradually begins to fade and 9 hours' after it is about the same degree of stain as that immediately after the addition of neutral red. It is almost completely faded after 24 hours' culture.
Published Date
1959-03-15
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume71
Issue
issue3-2
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
1537
End Page
1543
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/71/3-2/71_3-2_1537/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma