このエントリーをはてなブックマークに追加
ID 18419
Eprint ID
18419
FullText URL
Thumnail 74_713.pdf 11.2 MB
Title Alternative
Experimental Studies on Mercury Poisoning with the Use of Hg(203)(NO(3))(2) Ⅲ. Changes in mercury combined with protein and its excretion after the administration of BAL
Author
Tadatomo, Atsuo
Abstract
With the purpose to find out the effect of BAL (2.3 Dimercaptopropanol) in mercury poisoning, a group of guinea pigs were divided into two gruops of A group (control) and B group (BAL injection). To A group 1,200,000 c.p.m./kg of radio isotope Hg(203)(NO(3))(2) (0.026 mg/kg of mercury) was injected into the dorsal muscle, and to B group the same dose of Hg(203)(NO(3))(2) was given in the same way and immediately 20 mg/kg of BAL was injected and the same dose for the six consecutive days thereafter. Then, for the period of one week following the injection of Hg(203)(NO(3))(2), the excretion of mercury in the urine and stool as well as the distribution of mercury combined with protein in the intracellular granules of kidney after one week of Hg(203)(NO(3))(2) injection were observed in order to see the antidotal effect of BAL, and obtained the following results. 1. With the control group, the observations conducted with the lapse of time after Hg(203)(NO(3))(2) injection have revealed that the excretion of mercury in the urine is greatest on the second day, the excreted amount being about 21 per cent of the total mercury excreted in the urine in the one week period of observation. This excretion decreases relatively rapidly thereafter, but a transient rise in the quantity of excreted mercury can be observed on the fourth day. As for the excretion of mercury in stool, it tends to increase from the first day to the fifth day of the observation showing the greatest amount of the excretion on the fifth day and after that it decreases relatively rapidly. 2. In the group injection with BAL, the excretion of mercury in the urine is markedly great on the second day, this amount being as much as about 31 per cent of the total mercury excreted during the one-week period of observation, and it decreases quite rapidly thereafter. On the other hand, the amount of mercury excreted in the stool increases gradually from the first day to the third day of observation, showing the greatest amount of the excretion on the third day which is two days earlier than that in the control, and it decreases relatively slowly thereafter. 3. In the control group, the sum of the total amounts of mercury excreted in the urine and stool for the period of one week is about 49.8 per cent of the mercury injected, and of this about 35.9 per cent is excreted in the urine and that in the stool about 13.9 per cent, proving that the amouut excreted in the urine to be as much as about 2.6 times that in the stool. 4. In the group injected with BAL, the sum of the total amounts of mercury excreted in the urine and stool for the one-week period is about 82.7 per cent of the mercury injected, and of this about 69.5 per cent is excreted in the urine and about 13.2 per cent in the stool, proving that the amount of mercury excreted in the urine is about 5.3 times that in the stool. From the findings described in sections 3 and 4, in the group B given BAL, the amount of mercury excreted in the urine during the one-week period is about 69.5 per cent of the injected mercury, whereas that in the stool is about 35.9 per cent in the control A group, proving that the BAL administration has a marked effect on the excretion of mercury in the urine. On the other hand, the amount of mercury excreted in the stool is 13.2 per cent in the B group given BAL as against about 13.9 per cent in the A group, control. This means that there is hardly any effect of BAL on the excretion of mercury in the stool. 5. In the observation of the B group given BAL, the amount of the mercury combined with protein in kidney tissue has been found to decrease markedly after the BAL injection, and the amount of mercury cmbined with protein per 1.0 mg. of the tissue in dry weight is about 1/6.1 of that in the control group. In addition, in the intracellular granules of kidney, the amount of mercury combined with protein decreases markedly 1/8.0~1/5.6 of that in the control group in such fractions as nucleus, mitochondria, microsomes, and soluble protein, and such a decrease is most marked in the soluble protein.
Published Date
1962-09-30
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume74
Issue
issue8-9
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
713
End Page
727
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/74/8-9/74_8-9_713/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma