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ID 31556
JaLCDOI
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Author
Ota, Kosuke
Ota, Zensuke
Shikata, Kenichi Kaken ID researchmap
Makino, Hirofumi
Abstract

In order to clarify the mechanism of proteinuria in diabetic nephropathy, ultrastructural changes of the glomerular basement membrane (GBM) in patients with diabetic nephropathy were examined by electron microscopy using our newly devised "tissue negative staining method". The normal human GBM showed a fine meshwork structure consisting of fibrils forming the small pores. The diameter of these pores was slightly smaller than that of human albumin molecules. The GBM in patients with diabetic nephropathy showed irregular thickening. At higher magnification, hitherto unknown cavities and tunnel structures, which were not seen in normal controls, were observed in the thickened GBM. In some portions, these cavities presented a honeycomb-like appearance. The diameters of the cavities and tunnels were far larger than the dimensions of albumin molecules. These enlarged structures are believed to allow serum protein molecules to pass through the GBM from the capillary lumen to the urinary space. These results suggest that the cause of massive proteinuria in diabetic nephropathy is the disruption of the size barrier of the GBM.

Keywords
glomerular basement membrane
diabetic nephropathy
tissue negative staining
nephrotic syndrome
ultrastructure
Amo Type
Article
Published Date
1993-08
Publication Title
Acta Medica Okayama
Volume
volume47
Issue
issue4
Publisher
Okayama University Medical School
Start Page
267
End Page
272
ISSN
0386-300X
NCID
AA00508441
Content Type
Journal Article
language
英語
File Version
publisher
Refereed
True
PubMed ID
Web of Science KeyUT