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ID 32632
JaLCDOI
FullText URL
Author
Ota, Zensuke
Shikata, Kenichi Kaken ID researchmap
Ota, Kosuke
Abstract

We developed a "tissue negative staining method" to observe the molecular-level ultrastructure in situ in any portion of the ultrathin sections routinely prepared for electron microscopy. This method was used in electron microscopy of the glomerular basement membranes (GBM). The GBM in patients with nephrotic syndrome was discovered to possess a tunnel structure, designated as "nephrotic tunnel", with lumen large enough to allow free passage of protein molecules. This tunnel seemed to be involved in the etiology of nephrotic syndrome. This new method appears to be applicable to a variety of purposes in biological studies.

Keywords
mechanism
proteinuria
nephrotic syndrome
electron microscopy
negative staining
Amo Type
Article
Published Date
1992-12
Publication Title
Acta Medica Okayama
Volume
volume46
Issue
issue6
Publisher
Okayama University Medical School
Start Page
483
End Page
487
ISSN
0386-300X
NCID
AA00508441
Content Type
Journal Article
language
英語
File Version
publisher
Refereed
True
PubMed ID
Web of Science KeyUT