| JaLCDOI | 10.18926/AMO/53335 |
|---|---|
| FullText URL | 69_2_71.pdf |
| Author | Hiramoto, Hiroki| Dansako, Hiromichi| Takeda, Midori| Satoh, Shinya| Wakita, Takaji| Ikeda, Masanori| Kato, Nobuyuki| |
| Abstract | Persistent infection with hepatitis C virus (HCV) often causes chronic hepatitis, and then shows a high rate of progression to liver cirrhosis and hepatocellular carcinoma. To clarify the mechanism of the persistent HCV infection is considered to be important for the discovery of new target(s) for the development of anti-HCV strategies. In the present study, we found that the expression level of annexin A1 (ANXA1) in human hepatoma cell line Li23-derived D7 cells was remarkably lower than that in parental Li23 cells, whereas the susceptibility of D7 cells to HCV infection was much higher than that of Li23 cells. Therefore, we hypothesized that ANXA1 negatively regulates persistent HCV infection through the inhibition of viral RNA replication. The results revealed that HCV production was significantly inhibited without a concomitant reduction in the amount of lipid droplets in the D7 cells stably expressing exogenous ANXA1. Further, we demonstrated that ANXA1 negatively regulated the step of viral RNA replication rather than that of viral entry in human hepatocytes. These results suggest that ANXA1 would be a novel target for the development of anti-HCV strategies. |
| Keywords | HCV annexin A1 Li23 cell line Li23-derived D7 cells HCV-JFH-1 |
| Amo Type | Original Article |
| Published Date | 2015-04 |
| Publication Title | Acta Medica Okayama |
| Volume | volume69 |
| Issue | issue2 |
| Publisher | Okayama University Medical School |
| Start Page | 71 |
| End Page | 78 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | 英語 |
| Copyright Holders | CopyrightⒸ 2015 by Okayama University Medical School |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 25899628 |
| Web of Sience KeyUT | 000353181700001 |
| Author | Ariumi, Yasuo| Kuroki, Misao| Dansako, Hiromichi| Abe, Kenichi| Ikeda, Masanori| Wakita, Takaji| Kato, Nobuyuki| |
|---|---|
| Published Date | 2010-04-01 |
| Publication Title | 岡山医学会雑誌 |
| Volume | volume122 |
| Issue | issue1 |
| Content Type | Journal Article |
| Author | Kuroki, Misao| Ariumi, Yasuo| Hijikata, Makoto| Ikeda, Masanori| Dansako, Hiromichi| Wakita, Takaji| Shimotohno, Kunitada| Kato, Nobuyuki| |
|---|---|
| Published Date | 2013-01-11 |
| Publication Title | Biochemical and Biophysical Research Communications |
| Volume | volume430 |
| Issue | issue2 |
| Content Type | Journal Article |
| Author | Kato, Nobuyuki| Sejima, Hiroe| Ueda, Youki| Mori, Kyoko| Satoh, Shinya| Dansako, Hiromichi| Ikeda, Masanori| |
|---|---|
| Published Date | 2014-03-13 |
| Publication Title | PLOS ONE |
| Volume | volume9 |
| Issue | issue3 |
| Content Type | Journal Article |
| Author | Dansako, Hiromichi| Ueda, Youki| Okumura, Nobuaki| Satoh, Shinya| Sugiyama, Masaya| Mizokami, Masashi| Ikeda, Masanori| Kato, Nobuyuki| |
|---|---|
| Published Date | 2015 |
| Publication Title | The FEBS journal |
| Content Type | Journal Article |
| Author | Ueda, Youki| Takeda, Midori| Mori, Kyoko| Dansako, Hiromichi| Wakita, Takaji| Kim, Hye-Sook| Sato, Akira| Wataya, Yusuke| Ikeda, Masanori| Kato, Nobuyuki| |
|---|---|
| Published Date | 2013-08-30 |
| Publication Title | PLOS ONE |
| Volume | volume8 |
| Issue | issue8 |
| Content Type | Journal Article |
| Author | Dansako, Hiromichi| |
|---|---|
| Published Date | 2004-09-30 |
| Publication Title | |
| Content Type | Thesis or Dissertation |
