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ID 18295
Eprint ID
18295
FullText URL
Thumnail 76_477.pdf 865 KB
Title Alternative
Studies on a simple method for leucocyte culture
Author
Tawara, Jutaro
Terasaka, Takashi
Sakano, Saizo
Nishii, Satoru
Sibata, Jun
Abstract
The normal healthy human blood was heparinized with one-tenth volume of 0.1 per cent heparin solution. Ten thousand units of penicillin and 10mg of streptomycin were added to each 100ml of the blood. The whole blood was distributed into culture tubes in amounts of 0.5ml or 1.0ml. The tubes were rubber-stoppered, inclined at 40~45 degrees against the level, and iucubated in static state at 37℃. A few days after incubation, the plasma and free blood cells in tubes were discarded and washed with PBS twice. Then fresh culture medium was placed into the tubes to maintain a number of cells remained adhered on the wall.
Published Date
1964-09-30
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume76
Issue
issue7-9
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
477
End Page
479
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/76/7-9/76_7-9_477/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma