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ID 31747
JaLCDOI
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Author
Wakiguchi, Hiroshi
Fujieda, Mikiya
Matsumoto, Kenji
Ohara, Yuji
Kuroiwa, Yoshio
Wakiguchi, Akiko
Shiraishi, Taisuke
Kurashige, Takanobu
Kitamura, Isamu
Abstract

Antibody activity, especially that involved in the reaction of antibody-dependent cell-mediated cytotoxicity (ADCC), of five commercially available human gammaglobulin preparations (standard, pepsin-treated, plasmin-treated, polyethylene glycol-fractionated and S-sulfonated gammaglobulin) was measured. All these gammaglobulin preparations had high titers of hemagglutination inhibition and neutralizing antibody against measles virus. In ADCC reaction, the pepsin-treated gammaglobulin preparation showed no antibody activity. The standard gammaglobulin preparation showed weak activity only when highly diluted. The remaining three preparations showed high activity. Though the S-sulfonated gammaglobulin preparation showed no activity in ADCC reaction, it showed high activity after reconversion by means of oxidation and reduction in vitro. The plasmin-treated gammaglobulin preparation showed greater activity than the polyethylene glycol-fractionated preparation of the optimal concentration. In ADCC tests using the plasmin-treated gammaglobulin preparation, K cell activity was strongly inhibited by Hg (thimerosal), while, in those using the standard gammaglobulin preparation, the activity was hardly influenced by Hg, suggesting that the low ADCC activity of the standard gammaglobulin preparation of high concentrations was due to the inhibitory effect of aggregated immunoglobulin G molecules.

Keywords
antibody-dependent cell-mediated cytotoxicity
measles
immunology
gammaglobulin preparation
Amo Type
Article
Published Date
1987-04
Publication Title
Acta Medica Okayama
Volume
volume41
Issue
issue2
Publisher
Okayama University Medical School
Start Page
71
End Page
79
ISSN
0386-300X
NCID
AA00508441
Content Type
Journal Article
language
英語
File Version
publisher
Refereed
True
PubMed ID
Web of Sience KeyUT