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ID 40720
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Title Alternative
Primary culture of human primary liver cancer tissues treated with or without transcatheter arterial embolization and establishment of a cell line
Author
Kusaka, Yasunori
Tokiwa, Takayoshi
Sato, Jiro
Tsuge, Hiroshi
Hamasaki, Keisuke
Mimura, Hisashi
Orita, Kunzo
Abstract
Primary culture of human primary liver cancer tissues treated with or without trancatheter arterial embolization (TAE) was perfomed with the following results. 1. The yield and viability were very low in cells from primary liver cancer tissues dissociated with enzymes. 2. Epithelial-like cells were found from TAE-treated cancer tissues at a ratio of 1/8 in both monolayer and explant culture and from TAE-nontreated tissues at a ratio of 4/12 in monolayer culture and 3/12 in explant culture. The AFP-producing capahity of these epithelial-like cells has been maintained from one week to one month in culture. 3. Cells derived from two TAE-nontreated cancer tissues were subculturable. One was established as a cholangiocellular carcinoma cell line. 4. No heterotransplantability of primary cultured cancer cells into nude mice was found.
Keywords
TAE
ヒト肝癌
初代培養
株化
Published Date
1989-02
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume101
Issue
issue1-2
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
131
End Page
139
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/101/1-2/101_1-2_131/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma