このエントリーをはてなブックマークに追加
ID 10604
Eprint ID
10604
FullText URL
Title Alternative
Glutamate Snsor Using L-Glutamate Oxidase and Its Application for Sensing GOT/GPT Activity
Author
Arima, Jiro
Shinohara, Hiroaki
Kusakabe, Hitoshi
Tanaka, Hidehiko
Abstract
L-Glutamate measurement and GOT/GPT assay was successful by H2O2 measurement using the L-glutamate oxidase with 4-aminoantipyrine / phenol method. But, in examination of oxigen electrode, immobilized L-glutamate oxidase at the cellulose to L-glutamate and GOT-GPT sensor, Lpglutamate measurement was used for the amperometric determination with non-fixed enzyme. On examination of electron mediator, response for L-glutamate was observed with each of the compounds ferricyane, ferrocene-COOH, ferrocene-MeOH, and benzoquinone. L-Glutamate was measured by carbon printed tip electrode the L-glutamate oxidase and ferricyane based on the principle of chronoamperometry. A linear calibration graph was obtained between 1mM and 30mM. These results suggest that L-glutamate oxidase is able to utilize to L-glutamate sensor, and that there is a strong possibility to put this sensor to sensing for GOT/GPT activity.
Keywords
L-glutamate ocidase
L-glutamate sensor
GOT/GPT sensor
Published Date
2002-02
Publication Title
岡山大学農学部学術報告
Publication Title Alternative
Scientific reports of the Faculty of Agriculture, Okayama University
Volume
volume91
Issue
issue1
Publisher
岡山大学農学部
Publisher Alternative
Faculty of Agriculture,Okayama University
Start Page
15
End Page
22
ISSN
0474-0254
NCID
AN00033029
Content Type
Departmental Bulletin Paper
language
日本語
File Version
publisher
Refereed
False
Eprints Journal Name
srfa