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ID 13793
Eprint ID
13793
FullText URL
Thumnail 106_837.pdf 1.74 MB
Title Alternative
Sustaining capacity of hemopoiesis by human stromal cells in bone marrow
Author
Ueki, Koubun
Abstract
A bone marrow coculturing system developed by M. Y.Gordon was modified for easier evaluation of the function of bone marrow stromal cells. In the method agar was not used, and cells were dried and stained directly on a 35-mm petri dish after culture. The sustaining capacity of hemopoiesis by human stromal cells in bone marrow obtained by this method was compared between eight young and five eldery healthy volunteers. Hemopoietic progenitor cells were obtained from young volunteers and cultures were prepared for seven days. These appeared 86±28 colonies from 10(5) non-adherent bone marrow cells on the sheet of stromal cells from the young volunteers and 89±27 colonies from the eldery. The colonies were classified into two types according to cytochemical appearance through peroxidase staining, but all of them proved to be composed of myeloid cells by enzyme-labeled antibody staining. Colonies were identified easily and accurately by the newly modified coculturing method. No difference in sustaining capacity of hemopoiesis by human stromal cells in bone marrow was found between the young and the eldery using this system.
Keywords
human marrow stromal cell
ability to sustain hemopoiesis
IL-6 production
aging
Note
原著
Published Date
1994-08
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume106
Issue
issue7-8
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
837
End Page
846
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/106/7-8/106_7-8_837/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/index.html
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma