このエントリーをはてなブックマークに追加
ID 40624
FullText URL
Title Alternative
The effects of calmodulin inhibitors (W-7, TFP) in cultured glioma cells
Author
Bukeo, Takuji
Abstract
The effects of calmodulin inhibitors [W-7: N-(6-aminohexyl)-5-chloro-1-naphthalensulfonamide-HCl, TFP: Trifluoperazine]; cell growth inhibition, morphologic changes, and alterations of flow cytometry DNA hisotgram were studied in cultured glioma cells. The cell lines used in this experiment were KC and KY, which had been established from human glioblastomas, and C-6 from a methylnitrosourea-induced rat glioma. A dose-dependent growth inhibition was found in cells exposed to calmodulin inhibitors (W-7, TFP). The morphologic changes of cells were manifested by a rounding of the cells with numerous fine electron dense granules in the cytoplasm as early as 3 hours following exposure to calmodulin inhibitors. The influence of calmodulin inhibitors on cell kinetics was analysed by flow cytometry which revealed an accumulation of cells in G(2)+M phases within 24 hours after treatment. These results, together with the previous reports, indicate that the effects of calmodulin inhibitors on cell growth may be due not only to the inhibition of DNA synthesis in the G(1)/S boundary phase but also to the inhibition of tubulin disassembly in G(2)+M phases.
Keywords
カルモデュリン阻害剤
培養グリオーマ細胞
細胞増殖抑制
DNAヒストグラム
Published Date
1990-04
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume102
Issue
issue3-4
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
465
End Page
483
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/102/3-4/102_3-4_465/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma