このエントリーをはてなブックマークに追加
ID 53007
FullText URL
Author
Shigeyasu, Kunitoshi
Tazawa, Hiroshi ORCID Kakenhi publons
Hashimoto, Yuuri
Mori, Yoshiko
Kuroda, Shinji
Urata, Yasuo
Goel, Ajay
Kagawa, Shunsuke ORCID Kakenhi
Fujiwara, Toshiyoshi ORCID Kakenhi
Abstract
Background Molecular-based companion diagnostic tests are being used with increasing frequency to predict their clinical response to various drugs, particularly for molecularly targeted drugs. However, invasive procedures are typically required to obtain tissues for this analysis. Circulating tumour cells (CTCs) are novel biomarkers that can be used for the prediction of disease progression and are also important surrogate sources of cancer cells. Because current CTC detection strategies mainly depend on epithelial cell-surface markers, the presence of heterogeneous populations of CTCs with epithelial and/or mesenchymal characteristics may pose obstacles to the detection of CTCs. Methods We developed a new approach to capture live CTCs among millions of peripheral blood leukocytes using a green fluorescent protein (GFP)-expressing attenuated adenovirus, in which the telomerase promoter regulates viral replication (OBP-401, TelomeScan). Results Our biological capturing system can image epithelial and mesenchymal tumour cells with telomerase activities as GFP-positive cells. After sorting, direct sequencing or mutation-specific PCR can precisely detect different mutations in KRAS, BRAF and KIT genes in epithelial, mesenchymal or epithelial–mesenchymal transition-induced CTCs, and in clinical blood samples from patients with colorectal cancer. Conclusions This fluorescence virus-guided viable CTC capturing method provides a non-invasive alternative to tissue biopsy or surgical resection of primary tumours for companion diagnostics.
Published Date
2014-05-28
Publication Title
Gut
Content Type
Journal Article
Related Url
http://ousar.lib.okayama-u.ac.jp/metadata/52963
http://ousar.lib.okayama-u.ac.jp/metadata/53854
language
英語
File Version
author
Refereed
True
DOI