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ID 34242
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Author
Aoki, Takahiro
Suzuka, Shinya
Yamashita, Hiroki
Tanaka, Masaru
Matsunaga, Sachi
Kokumai, Shuhei
Abstract

Many plant retrotransposons have been characterized, but only three families (Tnt1, Tto1 and Tos17) have been demonstrated to be transpositionally competent. We followed a novel approach that enabled us to identify an active element of the Ty1-copia retrotransposon family with estimated 400 copies in the sweetpotato genome. DNA sequences of Ty1 -copia reverse transcriptase (RTase) from the sweetpotato genome were analyzed, and a group of retrotransposon copies probably formed by recent transposition events was further analyzed. 3’RACE on callus cDNA amplified transcripts containing long terminal repeats (LTR) of this group. The sequence -specific amplification polymorphism (S-SAP) patterns of the LTR sequence in the genomic DNA were compared between a normal plant and callus lines derived from it. A callus -specific S-SAP product was found into which the retrotransposon detected by the 3’RACE had been transposed apparently during cell culture. We conclude that our approach provides an effective way to identify active elements of retrotransposons with high copy numbers.

Keywords
retrotransposon
Ipomoea batatas
S-SAP
transposition
Note
Digital Object Identifer:10.1007/s00438-004-1044-2
Published with permission from the copyright holder. This is the institute's copy, as published in the Journal of Molecular Genetics and Genomics, August 2004, Volume 272, Issue 1, Pages 116-127.
Publisher URL:http://dx.doi.org/10.1007/s00438-004-1044-2
Direct access to Thomson Web of Science record
Copyright © 2004 Springer-Verlag. All rights reserved.
Published Date
2004-8
Publication Title
Molecular Genetics and Genomics
Volume
volume272
Issue
issue1
Start Page
116
End Page
127
Content Type
Journal Article
language
英語
Refereed
True
DOI
Web of Sience KeyUT
Submission Path
biochemistry/4