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ID 21134
Eprint ID
21134
FullText URL
Title Alternative
Studies on Rat Peritoneal Mast Cells 1. Purification of Mast Cells From Rat Peritoneal Cavity
Author
Tanizaki, Yoshiro
Tanaka, Juntaro
Komagoe, Haruki
Morinaga, Hiroshi
Kishimoto, Takumi
Kimura, Ikuro
Townley Robert G.
Abstract
To get a high recovery rate of mast cells from the rat peritoneal cavity, a new modified method was discussed using BSA density gradient. Three procedures, peritoneal lavage, washing of peritoneal cells and purification by BSA, were tested in this study, and the numbers of total cells and mast cells in each procedure were calculated. The recovery rate was the highest in BSA density gradient purification of the cells washed once with low speed centrifugation. The recovery rate was 73.7% and the number of mast cells taken from one rat was approximately 1.5×10(6). The purity of the cells with this procedure was 95.0% (range from 93.0 to 96.0%).
Note
原著論文 (Original Papers)
Published Date
1982-03-25
Publication Title
岡山大学温泉研究所報告
Publication Title Alternative
Papers of the Institute for Thermal Spring Research, Okayama University
Volume
volume52
Publisher
岡山大学温泉研究所
Publisher Alternative
Institute for Thermal Spring Research, Okayama University
Start Page
23
End Page
28
ISSN
0369-7142
NCID
AN00032853
Content Type
Departmental Bulletin Paper
language
日本語
File Version
publisher
Refereed
False
Eprints Journal Name
mmc