このエントリーをはてなブックマークに追加
ID 30753
JaLCDOI
FullText URL
Author
Nakatani, Satoru
Naito, Ichiro
Hinenoya, Noriko
Horiuchi, Kanji
Abstract

We attempted to prepare colloidal iron within tissues by means of microwave irradiation. Mouse tissue blocks were fixed with a mixture of paraformaldehyde and ferric chloride in a cacodylate buffer, immersed in a cacodylate buffered ferric chloride solution, and irradiated in a microwave processor. Colloidal iron was prepared within tissues or cells, and was observed in the form of electron dense fine granules (1-2 nm in diameter) by transmission electron microscopy. Collagen fibrils in the connective tissue showed colloidal iron deposition at regular periodical intervals. Cells in the splenic tissue showed that fine colloidal granules were deposited on the ribosomes but not on the nuclear chromatin. This finding suggests that ferric ions could not diffuse into the nucleus, which was surrounded by the nuclear envelope. The podocyte processes of the renal glomerulus were stained diffusedly. Though this microwave in situ colloidal iron preparation method has some limitations, it is convenient for use in biomedical specimen preparation in transmission electron microscopy.

Keywords
colloidal iron
microwave
histochemistry
transmission electron microscopy
Amo Type
Article
Published Date
2006-02
Publication Title
Acta Medica Okayama
Volume
volume60
Issue
issue1
Publisher
Okayama University Medical School
Start Page
59
End Page
64
ISSN
0386-300X
NCID
AA00508441
Content Type
Journal Article
language
英語
File Version
publisher
Refereed
True
PubMed ID
Web of Sience KeyUT