Author 百田 龍輔|
Published Date 2001-03-25
Publication Title
Content Type Thesis or Dissertation
JaLCDOI 10.18926/AMO/30753
FullText URL fulltext.pdf
Author Nakatani, Satoru| Naito, Ichiro| Momota, Ryusuke| Hinenoya, Noriko| Horiuchi, Kanji| Nishida, Keiichiro| Ohtsuka, Aiji|
Abstract <p>We attempted to prepare colloidal iron within tissues by means of microwave irradiation. Mouse tissue blocks were fixed with a mixture of paraformaldehyde and ferric chloride in a cacodylate buffer, immersed in a cacodylate buffered ferric chloride solution, and irradiated in a microwave processor. Colloidal iron was prepared within tissues or cells, and was observed in the form of electron dense fine granules (1-2 nm in diameter) by transmission electron microscopy. Collagen fibrils in the connective tissue showed colloidal iron deposition at regular periodical intervals. Cells in the splenic tissue showed that fine colloidal granules were deposited on the ribosomes but not on the nuclear chromatin. This finding suggests that ferric ions could not diffuse into the nucleus, which was surrounded by the nuclear envelope. The podocyte processes of the renal glomerulus were stained diffusedly. Though this microwave in situ colloidal iron preparation method has some limitations, it is convenient for use in biomedical specimen preparation in transmission electron microscopy.</p>
Keywords colloidal iron microwave histochemistry transmission electron microscopy
Amo Type Article
Published Date 2006-02
Publication Title Acta Medica Okayama
Volume volume60
Issue issue1
Publisher Okayama University Medical School
Start Page 59
End Page 64
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 16508690
Web of Sience KeyUT 000235538900007
JaLCDOI 10.18926/AMO/32859
FullText URL fulltext.pdf
Author Murakami, Shinichiro| Fujino, Hidemi| Takeda, Isao| Momota, Ryusuke| Kumagishi, Kanae| Ohtsuka, Aiji|
Abstract <p>The skeletal muscle is classified into 2 types, slow oxidative or fast glycolytic muscle. For further characterization, we investigated the capillary architecture in slow and fast muscles. The rat soleus and extensor digitorum longus (EDL) muscles were used as representatives of slow and fast muscles, respectively. To investigate capillary density, sections of both types of muscle were stained with alkaline phosphatase;the soleus muscle showed more intense reactivity, indicating that it had a denser capillary structure than the EDL muscle. We then injected fluorescent contrast medium into samples of both muscle types for light and confocal-laser microscopic evaluation. The capillary density and capillary-to-fiber ratio were significantly higher, and the course of the capillaries was more tortuous, in the soleus muscle than in the EDL muscle. Capillary coursed more tortuously in the soleus than in the EDL muscle. Succinate dehydrogenase (SDH) activity, an indicator of mitochondrial oxidative capacity, and vascular endothelial growth factor (VEGF) expression were also significantly higher in the soleus muscle. Thus, we conclude that slow oxidative muscle possess a rich capillary structure to provide demanded oxygen, and VEGF might be involved in the formation and/or maintenance of this highly capillarized architecture.</p>
Keywords skeletal muscle capillaly succinate dehydrogenase activity vascular endothelial growth factor
Amo Type Original Article
Published Date 2010-02
Publication Title Acta Medica Okayama
Volume volume64
Issue issue1
Publisher Okayama University Medical School
Start Page 11
End Page 18
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 20200579
Web of Sience KeyUT 000274868300002
Author Momota, Ryusuke| Naito, Ichiro| Ninomiya, Yoshifumi| Ohtsuka, Aiji|
Published Date 2011-05
Publication Title Matrix Biology
Volume volume30
Issue issue4
Content Type Journal Article
Author Shinaoka, Akira| Momota, Ryusuke| Shiratsuchi, Eri| Kosaka, Mitsuko| Kumagishi, Kanae| Nakahara, Ryuichi| Naito, Ichiro| Ohtsuka, Aiji|
Published Date 2013-04
Publication Title Microscopy and Microanalysis
Volume volume19
Issue issue2
Content Type Journal Article