岡山医学会 Acta Medica Okayama 0030-1558 72 1 1959 昇汞の消毒作用並びに毒性に関する研究 第2編 昇汞の毒性並びに昇汞中毒に対する6,8-Dithiooctanoic Acidの効果に就いて 131 138 EN Asao Sakai For the purpose of determining the toxicity of mercuric chloride the author performed experiments to find out LD(50) of this compound with the mice given intraperitoneal injection of mercuric chloride solution and also experiments on the antidotal effect of 6.8-dithiooetanoic acid: and obtained the following results. 1. The LD(50) values determined by Van den Waerdens method are as follows: 1 day LD(50)=0.01116±0.00102mg 2 day LD(50)=0.00997±0.001036mg 3 day LD(50)=0.00837±0.00108mg 4 day LD(50)=0.00712±0.00109mg 5 day LD(50)=0.0064±0.00109mg 6 day LD(50)=0.00574±0.00125mg 2. When 6.8-dithiooctanoic acid is injected to mice prior to intraperitoneal injection of the mercuric chloride solution, it has been found that fatality rate and toxic symptoms (adecrease in the erythrocyte count and an increase in the leucocyte count) are somewhat suppressed. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 72 1 1959 昇汞の消毒作用並びに毒性に関する研究 第1編 酸素消費量より見たる細菌に対する昇汞の消毒作用並びに大腸菌(E.Coii communis)の同位元素Hg(203)Cl(2)の吸着作用に就いて 123 130 EN Asao Sakai The auther studied the influence of mercuric chloride on the respiration of bacteria and the adsorption action of Hg(203)Cl(2) on E. coli eommunis; and obtained the following results. 1. The mercuric chloride action on the respiration of bacteria: a. The action of mercuric chloride acting upon the oxygen consumption of 10 mg by Coli communis begins to decrease at 5×10(-5)%, while it is completely stopped at 5×10(-4)%. b. The action of mercuric chloride acting on the oxygen consumption of 10 mg by Typhus abdominalis 57 S begins to decrease at 5×10(-4) and it almost disappears at 5×10(-2)%. c. The action of mercuric chloride on the oxygen consumption of 30 mg by Staph. aureus (Terajima strain) commences to diminish at 5×10(-5)% , and it disappears almost completely at 10(-2)%. 2. The action of radio-isotope Hg(203)C1(2) on the respiration of E. coli eommunis: a. It has been confirmed that clearly Hg ions of the mercuric chloride solution adsorb to E. coil communis. b. In the case where the culture time of Coli communis is prolonged, the adsorption of Hg ions has been found to rise clearly in proportion to the lapse of the time. c. In the case where the density of the mercuric chloride solution is altered, it has been recognized that the higher the concentration of the mercuric chloride solution, the higher the adsorption rate of ions would be. No potential conflict of interest relevant to this article was reported.