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ID 31638
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Author
Inamura, Keiji
Tahara, Seiji
Kurabayashi, Yuzuru
Akagi, Tadaatsu
Togami, Izumi
Takemoto, Mitsuhiro
Honda, Osamu
Morioka, Yasuki
Kawasaki, Shoji
Hiraki, Yoshio
Abstract

We developed a reliable system for the irradiation of xenografted tumors in mice which allows for accurate local irradiation under specific pathogen-free conditions. The system presented here consists of acrylic supports for mice and an acrylic box connected to a pump through 0.22 microns pore-sized filters. Mice with xenotransplanted tumors growing on their right hind legs were set on the supports and put into the box in a laminar flow hood. The tumors of 7 mice were irradiated simultaneously with X-rays of 6 and 10 MV generated by a linear accelerator at a dose rate of 3.1-4.7 Gy/min. The air was ventilated through filters during irradiation in the closed box. Microorganism tests confirmed that no bacteria entered or left the box. One of the significant characteristics of this setup is that it allows for irradiation under conditions of acute hypoxia, which is obtained using an integrated tourniquet. The dose variation among 7 tumors was less than 1%. The rest of the mouse's body was shielded effectively by a half-field technique and a lead block. As a result, the whole body dose for the mice was 0-4% of the total dose absorbed by the tumor. Due to the high dose rate and the ability to irradiate 7 mice simultaneously under specific pathogen-free conditions, this new system can be considered a time-saving and valuable tool for radiation oncology research.

Keywords
animal experiment
mouse
radiotherapy
linear accelerator
specirfic pathogen-free
Amo Type
Article
Published Date
1999-06
Publication Title
Acta Medica Okayama
Volume
volume53
Issue
issue3
Publisher
Okayama University Medical School
Start Page
111
End Page
118
ISSN
0386-300X
NCID
AA00508441
Content Type
Journal Article
language
英語
File Version
publisher
Refereed
True
Web of Sience KeyUT