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ID 48566
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Author
Kuroda, Masahiro Kaken ID researchmap
Katashima, Kazunori
Ashida, Masakazu
Matsuzaki, Hidenobu Kaken ID
Asaumi, Junichi Kaken ID researchmap
Ohno, Seiichiro
Kato, Hirokazu
Kanazawa, Susumu Kaken ID
Abstract
The roles of cell density, extracellular space, intracellular factors, and apoptosis induced by the molecularly targeted drug rituximab on the apparent diffusion coefficient (ADC) values were investigated using bio-phantoms. In these bio-phantoms, Ramos cells (a human Burkittセs lymphoma cell line) were encapsulated in gellan gum. The ADC values decreased linearly with the increase in cell density, and declined steeply when the extracellular space became less than 4 μm. The analysis of ADC values after destruction of the cellular membrane by sonication indicated that approximately 65% of the ADC values of normal cells originate from the cell structures made of membranes and that the remaining 35% originate from intracellular components. Microparticles, defined as particles smaller than the normal cells, increased in number after rituximab treatments, migrated to the extracellular space and significantly decreased the ADC values of bio-phantoms during apoptosis. An in vitro study using bio-phantoms was conducted to quantitatively clarify the roles of cellular factors and of extracellular space in determining the ADC values yielded by tumor cells and the mechanism by which apoptosis changes those values.
Keywords
apparent diffusion coefficient value
cell density
extracellular space
bio-phantom
Amo Type
Original Article
Published Date
2012-06
Publication Title
Acta Medica Okayama
Volume
volume66
Issue
issue3
Publisher
Okayama University Medical School
Start Page
263
End Page
270
ISSN
0386-300X
NCID
AA00508441
Content Type
Journal Article
language
英語
Copyright Holders
CopyrightⒸ 2012 by Okayama University Medical School
File Version
publisher
Refereed
True
PubMed ID
Web of Science KeyUT