JaLCDOI |
10.18926/AMO/31563
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FullText URL |
fulltext.pdf
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Author |
Nouso, Kazuhiro|
Battula, Narayama|
Thorgeirsson, Snorri S|
Higashi, Toshihiro|
Tsuji, Takao|
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Abstract |
<p>We expressed mouse cytochrome P1-450 and P3-450 using recombinant vaccinia virus gene expression system in HeLa cells that were devoid of significant basal levels of P-450. HeLa cells were infected with the recombinant vaccinia virus containing either mouse cytochrome P1-450 or P3-450 cDNA, and the cell lysates were analyzed for the kinetics of P-450 enzyme activity and protein expression at the same time. 7-Ethoxycoumarin O-deethylase and ethoxyresorufin O-deethylase activities were measured as an expression of the cytochrome P-450 enzyme activities. Both cell lines began to express these enzyme activities as early as 12h after infection. The activities increased linearly up to the 24 h time point, and were kept for 36 h. Western immunoblot analysis showed that these cytochrome P-450 proteins were detected at 16 h and reached maximum quantity at 24 h after infection. These data showed a good correlation between cytochrome P-450 enzyme activity and protein concentration throughout the process of P-450 gene expression by vaccinia virus vector, suggesting a complete formation of cytochrome P-450 holoenzyme from the early stage of the protein expression.</p>
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Keywords |
cytochrome P-450
vaccinia virus
kinetics
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Amo Type |
Article
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Published Date |
1993-12
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Publication Title |
Acta Medica Okayama
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Volume |
volume47
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Issue |
issue6
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Publisher |
Okayama University Medical School
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Start Page |
377
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End Page |
382
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ISSN |
0386-300X
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NCID |
AA00508441
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Content Type |
Journal Article
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language |
英語
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File Version |
publisher
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Refereed |
True
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PubMed ID |
8128911
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Web of Sience KeyUT |
A1993MP00700004
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