| JaLCDOI | 10.18926/AMO/31563 |
|---|---|
| FullText URL | fulltext.pdf |
| Author | Nouso, Kazuhiro| Battula, Narayama| Thorgeirsson, Snorri S| Higashi, Toshihiro| Tsuji, Takao| |
| Abstract | <p>We expressed mouse cytochrome P1-450 and P3-450 using recombinant vaccinia virus gene expression system in HeLa cells that were devoid of significant basal levels of P-450. HeLa cells were infected with the recombinant vaccinia virus containing either mouse cytochrome P1-450 or P3-450 cDNA, and the cell lysates were analyzed for the kinetics of P-450 enzyme activity and protein expression at the same time. 7-Ethoxycoumarin O-deethylase and ethoxyresorufin O-deethylase activities were measured as an expression of the cytochrome P-450 enzyme activities. Both cell lines began to express these enzyme activities as early as 12h after infection. The activities increased linearly up to the 24 h time point, and were kept for 36 h. Western immunoblot analysis showed that these cytochrome P-450 proteins were detected at 16 h and reached maximum quantity at 24 h after infection. These data showed a good correlation between cytochrome P-450 enzyme activity and protein concentration throughout the process of P-450 gene expression by vaccinia virus vector, suggesting a complete formation of cytochrome P-450 holoenzyme from the early stage of the protein expression.</p> |
| Keywords | cytochrome P-450 vaccinia virus kinetics |
| Amo Type | Article |
| Published Date | 1993-12 |
| Publication Title | Acta Medica Okayama |
| Volume | volume47 |
| Issue | issue6 |
| Publisher | Okayama University Medical School |
| Start Page | 377 |
| End Page | 382 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | 英語 |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 8128911 |
| Web of Sience KeyUT | A1993MP00700004 |
