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ID 308
Eprint ID
308
FullText URL
Title Alternative
シクロヘキシミドのタバコ葉核酸代謝におよぼす影響
Author
Ouchi, Seiji
Furusawa, Iwao
Akai, Shigeyasu
Abstract
The effect of cycloheximide on RNA metabolism in tobacco leaf discs was analyzed to signify its inhibitive or stimulative effect on TMV multiplication. RNA content increased in the leaf discs incubated in 1.0 or 3.0 ppm solution. 32P-incorporation into RNA, however, was accelerated at 1.0 ppm and disturbed at 3.0 ppm, suggestirg th~t the increment of RNA in the leaf disc<- treated at 1. O ppm is qualitatively different from that in the discs incubated at 3.0 ppm, i. e., the former is the result of stimulated synthesis while the latter is mainly due to the inhibition of degradation. Column chromatograms with methylated albumin Kiselguhr clearly elucidated that incubation of discs in 3.0 ppm led to the abolition of the integrity of the light ribosomal RNA. The change in chromatographic pattern was discernible 24 hr after the initiation of treatment of leaf discs. Incubation in 1.0 ppm cycloheximide, however, did not give rise to a drastic change in chromatograms, although an undulate incorporation of 32P-orthophosphate into ribosomal RNAs was noticed. 14C-glycine incorporation into RNA suggested that cycloheximide not only enhances RNA synthesis but also stimulates the synthesis of purine bases. Ribonuclease activity of leaf homogenate remarkably decreased by cycloheximide treatment, suggesting that the accumulation of RNA in the discs treated at 3.0 ppm could be acounted for by the retardation in RNA degradation. The inhibitory and stimulative effects of cycloheximide on TMV multiplication were discussed on the basis of these results.
Published Date
1972
Publication Title
岡山大学農学部学術報告
Publication Title Alternative
Scientific Reports of the Faculty of Agriculture Okayama University
Volume
volume40
Issue
issue1
Publisher
岡山大学農学部
Publisher Alternative
Faculty of Agriculture, Okayama University
Start Page
9
End Page
18
ISSN
0474-0254
NCID
AN00033029
Content Type
Departmental Bulletin Paper
language
英語
File Version
publisher
Refereed
False
Eprints Journal Name
srfa