JaLCDOI 10.18926/AMO/32623
FullText URL fulltext.pdf
Author Furuno, Katsushi| Gomita, Yutaka| Yoshida, Toshiko| Oishi, Ryozo| Saeki, Kiyomi| Araki, Yasunori|
Abstract <p>The plasma concentration of indomethacin was measured after the rectal administration of water-soluble and fatty base suppositories in rats. The results were compared with the in vitro indomethacin release from suppositories determined by Paddle method using three different types of membranes: cellulose membrane, artificial sausage membrane and natural sausage membrane. The plasma concentrations of indomethacin during the first 4h after the rectal administration were higher in rats that received water-soluble base suppositories than in those that received fatty base types. When either a cellulose membrane or an artificial sausage membrane of cow protein was used in the Paddle method, the amount of indomethacin released from fatty base suppositories was significantly higher than that from water-soluble base ones. However, the results were reversed when a natural sausage membrane of pig colon was used. The discrepancy in the in vitro experiments using water-soluble base suppositories seemed to be due to the difference of pore size of membrane used. Careful consideration should be given to the membrane used in the Paddle method especially when this method is employed to examine the release of poorly soluble drugs like indomethacin in both water-soluble and fatty base suppositories.</p>
Keywords indomethacin suppository in vitro cellulose membrane sausage membrane in vivo bioavailability
Amo Type Article
Published Date 1992-08
Publication Title Acta Medica Okayama
Volume volume46
Issue issue4
Publisher Okayama University Medical School
Start Page 223
End Page 231
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 1442146
Web of Sience KeyUT A1992JL44200001
JaLCDOI 10.18926/AMO/32186
FullText URL fulltext.pdf
Author Furuno, Katsushi| Gomita, Yutaka| Araki, Yasunori| Fukuda, Tamotu|
Abstract <p>We studied the use of high-performance liquid chromatography (HPLC), using a solid phase extraction column (Bond Elut cartridge column), for the simple, rapid and sensitive determination of serum clonazepam levels in epileptic patients. Extracted aliquots were analyzed by HPLC, using a reverse phase ODS column (mu-Bondapak C18). The analytical mean recovery of clonazepam added to the blank serum averaged 99.9%. The detection limit was as high as approximately 2 ng/ml in the serum. The reproducibilities were 2.3-8.6 CV % in the within-day assay and 6.5 CV % in the between-day assay, indicating that the analysis method was effective in the determination of clonazepam serum levels. Accordingly, we suggest that the present method, using a solid phase extraction column, may be useful for the routine monitoring of clonazepam serum levels in epileptic patients.&#60;/P&#62;</p>
Keywords clonazepam serum levels epileptic patient therapeutic drug monitoring solid-phase extraction HPLC
Amo Type Article
Published Date 1991-04
Publication Title Acta Medica Okayama
Volume volume45
Issue issue2
Publisher Okayama University Medical School
Start Page 123
End Page 127
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 1867113
Web of Science KeyUT A1991FL60800009
JaLCDOI 10.18926/AMO/31529
FullText URL fulltext.pdf
Author Fukuda, Tamotsu| Kawakami, Yasuhiro| Furuno, Katsushi| Araki, Yasunori|
Abstract <p>The onset of beta-methyl-digoxin action was investigated by the potentiation of the adenosine response in guinea pigs and rats, and compared with that of digoxin and dipyridamole. A number of i.v. infusions of adenosine were given to determine the mean control adenosine response and its 95% confidence limits. After oral administration of the drugs, successive infusions of adenosine were continued until a drug-induced potentiation of the adenosine response was observed. The time of appearance of the potentiated adenosine response was marked as the onset of action of the drugs. The onset of action in guinea pigs was 9 to 12 min for 0.2 to 0.4 mg/kg of beta-methyl-digoxin, 90 to 100 min for 0.2 mg/kg of digoxin and 25 min for 5 mg/kg of dipyridamole. The maximal potentiation was 48.8 to 53.8% at 18 to 21 min for beta-methyl-digoxin, 74.5% at 130 min for digoxin and 74.8% at 80 min for dipyridamole. Adenosine infused i.v. into rats produced heart block, as in guinea pigs. However, in rats, the adenosine response was not potentiated by beta-methyl-digoxin and digoxin. Dipyridamole at a dose as high as 200 mg/kg produced 25.8% potentiation at 36 min after oral administration to rats.</p>
Keywords ?-methy1-digoxin digoxin dipyridamole onset of action guinea pigs and rats
Amo Type Article
Published Date 1985-06
Publication Title Acta Medica Okayama
Volume volume39
Issue issue3
Publisher Okayama University Medical School
Start Page 171
End Page 177
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 4024991
Web of Sience KeyUT A1985ALG3300002
JaLCDOI 10.18926/AMO/30468
FullText URL fulltext.pdf
Author Gomita, Yutaka| Furuno, Katsushi| Araki, Yasunori|
Abstract <p>The plasma level of isosorbide dinitrate intraperitoneally administered to rats stressed by foot-shock was almost the same as that in non-stressed control rats. However, levels of its metabolites, 5-isosorbide mononitrate and 2-isosorbide mononitrate, were lower in stressed rats than in non-stressed rats, suggesting that stress may influence the metabolism and/or excretion of the metabolites.</p>
Keywords isosorbird dinitrate pharmacokinetics emotional stress rats
Amo Type Article
Published Date 1990-02
Publication Title Acta Medica Okayama
Volume volume44
Issue issue1
Publisher Okayama University Medical School
Start Page 51
End Page 53
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 2330846
Web of Science KeyUT A1990CT06800008
JaLCDOI 10.18926/AMO/30402
FullText URL fulltext.pdf
Author Watanabe, Kazuhide| Eto, Koehi| Furuno, Katsushi| Mori, Takaaki| Kawasaki, Hiromu| Gomita, Yutaka|
Abstract <p>The effect of cigarette smoke on organ weights, lipid peroxidation and plasma biochemical parameters was investigated in male Wistar rats. Daily exposure (for 20 min twice a day) to cigarette smoke for 27 days caused a significant decrease in liver weight and a significant increase in lung weight. The smoke-exposure group showed increased lipid peroxidation in the liver, but not in the lung. In the smoke-exposure group, the GOT, gamma-GTP, total bilirubin and LDH values were significantly higher than those in the control group, while the plasma glucose value was significantly lower. These results suggest that cigarette smoking might induce liver injury by enhancing lipid peroxidation.</p>
Keywords cigarette smoking lipid peroxidation liver function rats
Amo Type Article
Published Date 1995-10
Publication Title Acta Medica Okayama
Volume volume49
Issue issue5
Publisher Okayama University Medical School
Start Page 271
End Page 274
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 8585399
Web of Science KeyUT A1995TC51800008
JaLCDOI 10.18926/AMO/30398
FullText URL fulltext.pdf
Author Moriyama, Masahiro| Domoto, Haruyo| Yamashita, Syoichi| Furuno, Katsushi| Oishi, Ryozo| Kawasaki, Hiromu| Gomita, Yutaka|
Abstract <p>We examined the pharmacokinetics of phenobarbital before and during pregnancy in rats. Animals were divided into four groups: (a) control, (b) pregnant, (c) phenobarbital-treated, and (d) phenobarbital-treated pregnant groups. The increase in body weight of nonpregnant or pregnant rats was not influenced by long-term phenobarbital treatment. Plasma phenobarbital concentrations during the period of long-term phenobarbital treatment with a fixed dosage by body weight were not significantly affected by pregnancy. Furthermore, pregnancy did not affect pharmacokinetic parameters of phenobarbital between 0.25 and 24h after administration. These results suggest that pregnancy does not influence on the pharmacokinetics of long-term phenobarbital treatment at a fixed dosage by body weight.</p>
Keywords phenobarbital pharmacokinetics pregnancy plasma concentrations
Amo Type Article
Published Date 1995-10
Publication Title Acta Medica Okayama
Volume volume49
Issue issue5
Publisher Okayama University Medical School
Start Page 237
End Page 240
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 8585393
Web of Science KeyUT A1995TC51800002
JaLCDOI 10.18926/AMO/30377
FullText URL fulltext.pdf
Author Furuno, Katsushi| Okazaki, Masatoshi| Eto, Kohei| Kawasaki, Hiromu| Gomita, Yutaka|
Abstract <p>The effects of acute exposure to cigarette smoke and systemic administration of nicotine on intestinal propulsion were investigated in rats. The propulsive activity was measured as migration of charcoal powder in the intestine. This activity was suppressed by acute exposure (10 min) to cigarette smoke and by nicotine (0.5 mg/kg x 2, s.c.) administration. This intestinal suppression was more marked in the rats given nicotine than in those exposed to cigarette smoke, whereas the plasma concentrations of nicotine in both rats were similar. These results suggest that acute exposure to cigarette smoke and nicotine administration delay gastric emptying and/or suppress intestinal propulsion, and that some components other than nicotine contained in cigarette smoke may attenuate the suppression of intestinal propulsion induced by nicotine.</p>
Keywords cigarette smoke nicotine intestinal propulsion
Amo Type Article
Published Date 1995-08
Publication Title Acta Medica Okayama
Volume volume49
Issue issue4
Publisher Okayama University Medical School
Start Page 201
End Page 204
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 7502680
Web of Science KeyUT A1995RR97800004