JaLCDOI 10.18926/AMO/30724
FullText URL Fulltext.pdf erratum_61_2_121.pdf
Author Fujita, Osamu| Asanuma, Masato| Yokoyama, Teruhiko| Miyazaki, Ikuko| Ogawa, Norio| Kumon, Hiromi|
Abstract We examined the involvement of the signal transducer and activator of transcription 3 (STAT3) in bladder outlet obstruction (BOO)-induced bladder smooth muscle hypertrophy using a rat in vivo and in vitro study. BOO induced increases in bladder weight and bladder smooth muscle thickness 1 week after the operation. By using antibody microarrays, 64 of 389 proteins blotted on the array met our selection criteria of an INR value between > or = 2.0 and < or = 0.5. This result revealed up-regulation of transcription factors, cell cycle regulatory proteins, apoptosis-associated proteins and so on. On the other hand, down-regulation (INR value < or = 0.5) of proteins was not found. In a profiling study, we found an increase in the expression of STAT3. A significant increase in nuclear phosphorylated STAT3 expression was confirmed in bladder smooth muscle tissue by immunohistochemistry and Western blot analysis. Cyclical stretch-relaxation (1 Hz) at 120% elongation significantly increased the expression of STAT3 and of alpha-smooth muscle actin in primary cultured bladder smooth muscle cells. Furthermore, the blockade of STAT3 expression by the transfection of STAT3 small interfering RNA (siRNA) significantly prevented the stretch-induced increase in alpha-smooth muscle actin expression. These results suggest that STAT3 has an important role in the induction of bladder smooth muscle hypertrophy.
Keywords benign prostatic hyperplasia bladder outlet obstruction bladder smooth muscle signal transducer and activator of transcription 3 (STAT3) small interfering RNA (siRNA)
Amo Type Original Article
Published Date 2006-12
Publication Title Acta Medica Okayama
Volume volume60
Issue issue6
Publisher Okayama University Medical School
Start Page 299
End Page 309
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 17189973
Web of Science KeyUT 000243019000001