JaLCDOI 10.18926/AMO/32291
FullText URL fulltext.pdf
Author Kamei, Kiseko| Matsuoka, Hiroyuki| Furuhata, Syun-ichi| Fujisaki, Ryu-ichi| Kawakami, Tomotaka| Mogi, Seiji| Yoshihara, Hisanao| Aoki, Naoko| Ishii, Akira| Shibuya, Toshihiro|
Abstract <p>To find a new anti-malarial medicine derived from natural resources, we examined the leaves of 13 common Japanese plants in vitro. Among them, a leaf-extract of Hydrangea macrophylla, a common Japanese flower, inhibited the parasitic growth of Plasmodium falciparum. The IC50 of Hydrangea macrophylla leaf extract to Plasmodium falciparum was 0.18 microg/ml. The IC50 to NIH 3T3-3 cells, from a normal mouse cell line, was 7.2 microg/ml. Thus, selective toxicity was 40. For the in vivo test, we inoculated Plasmodium berghei, a rodent malaria parasite, to ddY mice and administered the leaf-extract of Hydrangea macrophylla (3.6 mg/0.2 ml) orally 3 times a day for 3 days. Malaria parasites did not appear in the blood of in the treated mice, but they did appear in the control group on day 3 or 4 after inoculation with the parasites. When leaf extract was administered to 5 mice 2 times a day for 3 days, malaria parasites did not appear in 4 of the mice but did appear in 1 mouse. In addition, the leaf-extract was administered orally 3 times a day for 3 days to Plasmodium berghei infected mice with a parasitemia of 2.7%. In the latter group, malaria parasites disappeared on day 3 after initiating the treatment, but they appeared again after day 5 or 6. Although we could not cure the mice entirely, we confirmed that the Hydrangea macrophylla leaf extract did contain an anti-malarial substance that can be administered orally.</p>
Keywords hydrangea macrophylla malaria medical plant plasmodium berghei plasmodium falciparum
Amo Type Article
Published Date 2000-10
Publication Title Acta Medica Okayama
Volume volume54
Issue issue5
Publisher Okayama University Medical School
Start Page 227
End Page 232
ISSN 0386-300X
NCID AA00508441
Content Type Journal Article
language 英語
File Version publisher
Refereed True
PubMed ID 11061572
Web of Science KeyUT 000090098600006